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001			233802
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008			100301s2001 xxu| s |||| 0|eng d
020			_a9781592592333
024	7		_a10.1385/1592592333 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNuclease Methods and Protocols _cedited by Catherine H. Schein.
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XVII, 525 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v160
505	0		_aCharacterizing Nuclease Activity -- Processivity of DNA Repair Enzymes -- Analysis by HPLC of Distributive Activities and the Synthetic (Back) Reaction of Pancreatic-Type Ribonucleases -- Ier1 p: A Kinase and Site-Specific Endoribonuclease -- Microtiter-Plate Assay and Related Assays for Nonspecific Endonucleases -- Quantitating mRNAs with Relative and Competitive RT-PCR -- Expressing Self-Incompatibility RNases (S-RNases) in Transgenic Plants -- Molecular Cloning, Tissue Distribution, and Chromosomal Localization of the Human Homolog of the R2/Th/Stylar Ribonuclease Gene Family -- Inhibitors and Activators of Nucleases -- Ribonuclease Inhibitors -- Producing Soluble Recombinant RNases and Assays to Measure Their Interaction with Interferon-? In Vitro? -- Assays for the Evaluation of HIV-1 Integrase Inhibitors -- The Direction of Ribonucleases H by Antisense Oligodeoxynucleotides -- The 2-5A/RNase L Pathway and Inhibition by RNase L Inhibitor (RLI) -- Relating Nucleas Structure and Function -- Crystallization and Crystal Structure Determination of Ribonuclease A-Ribonuclease Inhibitor Protein Complex -- Methods for Studying the Interaction of Barnase with Its Inhibitor Barstar -- Assaying In Vitro Refolding of RNases by Mass Spectrometry -- Dissecting Nucleases into Their Structural and Functional Domains -- Using Electrostatics to Define the Active Site of Serratia Endonuclease -- Homology Modeling and Simulations of Nuclease Structures -- Methods for Determining Activity and Specificity of DNA Binding and DNA Cleavage by Class II Restriction Endonucleases -- Engineered Properties and Assays for Human DNase I Mutants -- Nucleases in the Clinic -- Assays for Human DNase I Activity in Biological Matrices -- Evaluation of Retroviral Ribonuclease H Activity -- Assays for Detection of RNase A Superfamily Ribonucleases -- Assay for Antitumor and Lectin Activity in RNase Homologs -- Isolation and Enzymatic Activity of Angiogenin -- Preparation and Preclinical Characterization of RNase-Based Immunofusion Proteins -- Assays Using Nucleases -- Restriction Endonucleases and Their Uses -- Isolation and Characterization of an Unknown Restriction Endonuclease -- Gene Modification with Hapaxoterministic Restriction Enzymes -- Techniques to Measure Nucleic Acid-Protein Binding and Specificity -- Analyzing the Developmental Expression of Sigma Factors with S1-Nuclease Mapping -- Detection and Quantitation of mRNAs Using Ribonuclease Protection Assays.
520			_aNucleases, the enzymes that cleave and shape nucleic acids, are key regulators of cellular metabolism. Angiogenesis and the unfolded protein response are just a few instances of important intra- and extracellular pathways regulated by nucleases. In Nuclease Methods and Protocols, researchers at the forefront of academic research and pharmaceutical drug development from four continents summarize their recent results. Their projects involve nucleases in many different areas of medicine and biology, ranging from protein structure and folding, to DNA repair, to developing cures for insidious diseases such as cystic fibrosis, AIDS, and cancer. Each chapter contains a minireview of a specific nuclease or nuclease-related theme, a discussion of why and when to use the assays described, and often, an explanation of how the assay was developed. The authors describe their most valuable methods for determining the mode of action, structure, interaction with other molecules, and physiological role of nucleases. Detailed step-by-step instructions and notes on avoiding pitfalls help ensure readily reproducible results. Comprehensive and highly practical, Nuclease Methods and Protocols offers both novice and experienced researchers a deeper understanding of the importance of nucleases in cell metabolism, growth control mechanisms, and, increasingly, in diagnostics and therapeutics.
700	1		_aSchein, Catherine H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371301
776	0	8	_iPrinted edition: _z9781489942272
776	0	8	_iPrinted edition: _z9780896036796
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592592333 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2001
999			_c233802 _d233802