000			03194nam a22003615i 4500
001			233805
003			ES-VaUE
005			20221220020522.0
007			cr nn 008mamaa
008			100301s2000 xxu| s |||| 0|eng d
020			_a9781592590384
024	7		_a10.1385/1592590381 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Nucleic Acid Protocols Handbook _cedited by Ralph Rapley.
250			_a1st edition 2000
264		1	_aTotowa, NJ _bHumana Press _c2000
300			_a1 recurso en línea (DLXXII, 478 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aSpringer Protocols Handbooks _x1949-2456
505	0		_aNucleic Acid Extraction -- Basic Separation and Analysis of DNA -- Probe Design, Synthesis, and Labeling -- RNA Analysis Techniques -- Gene Library Construction and Screening -- DNA Sequencing -- Basic Polymerase Chain Reaction Methods -- Analyzing Genes, Mutations, and Protein Interactions -- mutagenesis, transcription, and Translation In Vitro -- Gene Localization, Mapping In Situ, and Bioinformatics.
520			_aThe Nucleic Acid Protocols Handbook is today's most comprehensive up-to-date treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular Biology™ series, providing an introduction to the scientific basis of each technique, a complete listing of all the materials and reagents needed, and clear step-by-step instructions to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success in the lab. The authord are well-experienced masters of the techniques described, often having perfected and optimized them in their own laboratories. In The Nucleic Acid Protocols Handbook, Ralph Rapley has brought together the widest-ranging, one-volume collection of classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids. Eminently practical and reproducible, these proven methods will help both experienced researchers and those entering the field attain the laboratory mastery needed-not only to deepen understanding of biological processes at the molecular level, but also to begin realizing the biotechnological bounty so clearly in prospect.
700	1		_aRapley, Ralph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9780896038417
776	0	8	_iPrinted edition: _z9781489941497
776	0	8	_iPrinted edition: _z9780896034594
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592590381 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2000
999			_c233805 _d233805