000			03932nam a22003615i 4500
001			233808
003			ES-VaUE
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007			cr nn 008mamaa
008			100301s2003 xxu| s |||| 0|eng d
020			_a9781592593934
024	7		_a10.1385/1592593933 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aLentivirus Gene Engineering Protocols _cedited by Maurizio Federico.
250			_a1st edition 2003
264		1	_aTotowa, NJ _bHumana Press _c2003
300			_a1 recurso en línea (XII, 316 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v229
505	0		_aLentivirus Vectors -- From Lentiviruses to Lentivirus Vectors -- The Choice of a Suitable Lentivirus Vector -- Choice and Use of Appropriate Packaging Cell Types -- Small- to Large-Scale Production of Lentivirus Vectors -- Detection and Titration of Lentivirus Vector Preparations -- Detection and Selection of Lentiviral Vector-Transduced Cells -- Detection of Replication-Competent Lentiviral Particles -- Transduction of EX Vivo Cell Cultures Through Lentivirus Vectors -- Lymphocytes -- Monocyte/Macrophages and Dendritic Cells -- Hematopoietic Stem and Progenitor Cells -- Mesenchymal Stem Cells -- Hippocampal Neurons -- Dorsal Root Ganglia Sensory Neurons -- Cardiomyocytes -- Airway Epithelia -- Corneal Cells -- Retinal Tissue -- Advantages and Uses of Non-HIV-1-Based Lentivirus Vectors -- HIV-2 Vectors -- SIV Vectors -- FIV Vectors -- Hybrid Lentivirus Vectors -- Direct In Vivo Transduction by Lentivirus Vector and Detection -- Cells of Respiratory Epithelium -- Cells of the Nervous System.
520			_aLentivirus vector-based technologies, through in vitro and in vivo gene transfer in eukaryotic animal cells, currently offer the most promising opportunities for curing genetic disorders, as well as cancer and infectious diseases. In Lentivirus Gene Engineering Protocols, Maurizio Federico has assembled a panel of outstanding experimenters to detail all the theoretical and practical aspects of lentivirus vector-based gene transfer. The authors demonstrate lentivirus vector production with detailed methods for recovering appropriate producer cells, for producing and titrating lentivirus-containing supernatants, and for detecting transduced cells. The applications of lentivirus vector engineering to different cell types include coverage of lymphocytes, dendritic cells, hematopoietic stem and progenitor cells, mesenchymal stem cells, hippocampal neurons, cardiomyocytes, as well as airway epithelia, corneal cells, and retinal pigment. The use of non-HIV-1-based lentivirus vectors is shown to be advantageous, particularly those obtained from the genomes of HIV-2, SIV, or FIV. Also advanced are applications in which genetic material is directly inoculated with lentivirus vectors in such experimental animal models as mouse, rat, and rabbit. Comprehensive and informative, Lentivirus Gene Engineering Protocols presents a broad spectrum of the latest methods of lentivirus vector technology, offering hematologists, neurologists, geneticists, and virologists the most promising and up-to-date tools available for gene therapy in such still fatal diseases as cancer, cystic fibrosis, and AIDS.
700	1		_aFederico, Maurizio _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617373510
776	0	8	_iPrinted edition: _z9781588290915
776	0	8	_iPrinted edition: _z9781489938183
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593933 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2003
999			_c233808 _d233808