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007			cr nn 008mamaa
008			150402s2015 xxu| s |||| 0|eng d
020			_a9781493924981
024	7		_a10.1007/978-1-4939-2498-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aT follicular Helper Cells _bMethods and Protocols _cedited by Marion Espéli, Michelle Linterman.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XI, 228 páginas) _b51 ilustraciones, 26 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1291
505	0		_aIdentification of Mouse T Follicular Helper Cells by Flow Cytometry -- Identification of Follicular T Helper Cells in Tissue Sections -- Tracking Early T Follicular Helper Cell Differentiation In vivo -- Tracking by Flow Cytometry Antigen-Specific Follicular Helper T-Cells In Wild-Type Animals After Protein Vaccination -- Retroviral Vector Expression in TCR Transgenic CD4+ T Cells -- Two-photon Microscopy For Imaging Germinal Centres and T Follicular Helper Cells -- In vivo Induction of T-follicular Helper Cells by Modulation of Regulatory T Cell Function -- Assessing T Follicular Helper Cell Function in vivo: Antigen-specific B Cell Response to Hapten and Affinity Maturation -- The SWHEL System For High-resolution Analysis of in vivo Antigen-specific T-Dependent B Cell Responses -- Triggering Positive Selection of Germinal Center B cells by Antigen Targeting to DEC-205 -- Detection of Mouse Natural Killer T Follicular Helper (NKTFH) Cells by Flow Cytometry -- Identification of Foxp3+ T Follicular Regulatory (Tfr) Cells by Flow Cytometry -- In vitro Assay to Sensitively Measure Tfr Suppressive Capacity and Tfh Stimulation of B Cell Responses -- Flow Cytometric Detection and Isolation of Human Tonsil or Lymph Node T Follicular Helper Cells -- Human Tfh and Tfr cells: Identification and Assessment of their Migration Potential -- Analysis of Human Blood Memory T Follicular Helper Subsets -- Flow Cytometric Analysis of Circulating Follicular Helper T (Tfh) and Follicular Regulatory T (Tfr) Populations in Human Blood -- Quantifying Helper Cell Function of Human TFH Cells in vitro.
520			_aThis volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans.  As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.   Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.
700	1		_aEspéli, Marion _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aLinterman, Michelle _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493924998
776	0	8	_iPrinted edition: _z9781493924974
776	0	8	_iPrinted edition: _z9781493955930
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2498-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c233815 _d233815