000			04038nam a22003615i 4500
001			233827
003			ES-VaUE
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007			cr nn 008mamaa
008			110414s2009 xxu| s |||| 0|eng d
020			_a9781597452021
024	7		_a10.1007/978-1-59745-202-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicroinjection _bMethods and Applications _cedited by David J. Carroll.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XVI, 214 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v518
505	0		_aExpression of Exogenous mRNA in Xenopus laevis Embryos for the Study of Cell Cycle Regulation -- Use of Luciferase Chimaera to Monitor PLC? Expression in Mouse Eggs -- Analysis of 14-3-3 Family Member Function in Xenopus Embryos by Microinjection of Antisense Morpholino Oligos -- A Microinjectable Biological System, the Xenopus Oocyte, as an Approach to Understanding Signal Transduction Protein Function -- Combining Microinjection and Immunoblotting to Analyze MAP Kinase Phosphorylation in Single Starfish Oocytes and Eggs -- Analysis of Signaling Pathways in Zebrafish Development by Microinjection -- Protein Inhibition by Microinjection and RNA-Mediated Interference in Tissue Culture Cells: Complementary Approaches to Study Protein Function -- DNA Delivery by Microinjection for the Generation of Recombinant Mammalian Cell Lines -- Bacteriophage ?C31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels -- Germline Transformation of Caenorhabditis elegans by Injection -- Quantitative Microinjection of Mouse Oocytes and Eggs -- Microinjection of Follicle-Enclosed Mouse Oocytes -- Functional Studies of Regulatory Genes in the Sea Urchin Embryo -- Exploring the Cytoskeleton During Intracytoplasmic Sperm Injection in Humans -- Somatic Cell Nuclear Transfer in the Mouse.
520			_aAs the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Applications, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and easily accessible, Microinjection: Methods and Applications is an ideal source for new ideas and techniques certain to aid in the advance of biological research.
700	1		_aCarroll, David J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781607610922
776	0	8	_iPrinted edition: _z9781588298843
776	0	8	_iPrinted edition: _z9781493960903
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-202-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c233827 _d233827