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008			100301s2001 xxu| s |||| 0|eng d
020			_a9781592590735
024	7		_a10.1385/159259073X _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAtherosclerosis _bExperimental Methods and Protocols _cedited by Angela F. Drew.
250			_a1st edition 2001
264		1	_aTotowa, NJ _bHumana Press _c2001
300			_a1 recurso en línea (XI, 247 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v52
505	0		_aAnimal Models of Diet-Induced Atherosclerosis -- Mechanical Injury Models -- Genetically Manipulated Models of Atherosclerosis in Mice -- Lipoprotein Isolation and Analysis from Serum by Preparative Ultracentrifugation -- Separation of Plasma Lipoproteins in Self-Generated Gradients of Iodixanol -- Fractionation of Lipoprotein Subclasses in Self-Generated Gradients of Iodixanol -- 7 Detecting Cholesteryl Ester Transfer Protein in Plasma -- 8 Determination of Plasma Homocysteine -- 9 Assay for Serum Glycated Lipoproteins -- 10 Lipoprotein(a) Quantitation -- Isolation and Characterization of Glycosaminoglycans from Human Atheromatous Vessels -- Culture of Human Smooth Muscle Cells -- Culture of Human Endothelial Cells -- Assay for Cell Migration and Invasion of Vascular Smooth Muscle Cells -- Collection and Processing of Arterial Specimens for Histological Analysis -- Intravascular Ultrasound Imaging in the Quantitation of Atherosclerosis in Vivo -- Identification of Cell Types and Quantification of Lesion Composition -- 1Nonradioactive In Situ Hybridization in Atherosclerotic Tissue -- Evaluation of Angiogenesis -- Evaluation of Cell Proliferation in Human Atherosclerotic Lesions -- Gene Transfer to the Vasculature Historical Perspective and Implications for Future Research Objectives -- A Pig Model of Vein Graft Disease Applications for Potential Gene Therapies.
520			_aAtherosclerosis-a major killer of people in Western societies-is not yet completely understood. In Atherosclerosis: Methods and Protocols, Angela Drew and a panel of experts have assembled a comprehensive collection of traditional and cutting-edge techniques for investigating this disease and its possible treatments. Each readily reproducible method includes step-by-step instructions and practical information regarding sample collection, the choice of animal model system, experimental design, and practical data analysis techniques. Assay systems for serum or plasma determination of lipoproteins are also described, along with assays for those plasma proteins recently identified as implicated in atherogenesis, including cholestryl ester transfer protein, homocysteine, glycated lipoproteins, and apolipoprotein(a). Additional techniques cover the isolation and culture of cells and glycosaminoglycans from atherosclerotic plaques, and the collection and analysis of experimental atherosclerotic lesions. Comprehensive and richly detailed, Atherosclerosis: Methods and Protocols enables all biomedical investigators to select those optimized techniques that may be most fruitfully used to study the development, progression, and treatment of atherosclerotic lesions today.
700	1		_aDrew, Angela F _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617371684
776	0	8	_iPrinted edition: _z9780896037519
776	0	8	_iPrinted edition: _z9781489942791
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/159259073X _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2001
999			_c233828 _d233828