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008			171124s2018 xxu| s |||| 0|eng d
020			_a9781493974542
024	7		_a10.1007/978-1-4939-7454-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBacterial Multidrug Exporters _bMethods and Protocols _cedited by Akihito Yamaguchi, Kunihiko Nishino.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 355 páginas) _b91 ilustraciones, 53 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1700
505	0		_aHigh Resolution Crystallographic Analysis of AcrB Using Designed Ankyrin Repeat Proteins (DARPins) -- Crystallographic Analysis of Drug and Inhibitor-Binding Structure of RND-Type Multidrug Exporter AcrB in Physiologically-Relevant Asymmetric Crystals -- Crystallographic Analysis of MATE-Type Multidrug Exporter with Its Inhibitors -- Crystallographic Analysis of the CusBA Heavy-Metal Efflux Complex of Escherichia coli.- Purification of AcrAB-TolC Multidrug Efflux Pump for Cryo-EM Analysis -- NMR Spectroscopy Approach to Study the Structure, Orientation, and Mechanism of the Multidrug Exporter EmrE -- Generation of Conformation-Specific Antibody Fragments for Crystallization of the Multidrug Resistance Transporter MdfA -- Biochemical Reconstitution and Characterization of Multi-Component Drug Efflux Transporters -- Covalently Linked Trimers of RND (Resistance-Nodulation-Division) Efflux Transporters to Study Their Mechanism of Action: Escherichia coli AcrB Multidrug Exporter as an Example -- Determining Ligand Path through a Major Drug Transporter, AcrB, in Escherichia coli.- Molecular Modeling of Multi-Drug Properties of Resistance Nodulation Division (RND) Transporters -- A Transcriptomic Approach to Identify Novel Drug Efflux Pumps in Bacteria -- Regulation of the Expression of Bacterial Multidrug Exporters by Two-Component Signal Transduction Systems -- Study of the Expression of Bacterial Multidrug Efflux Pumps in Anaerobic Conditions -- Identification of a Staphylococcus aureusEfflux Pump Regulator Using a DNA-Protein Affinity Technique -- High Throughput Flow Cytometry Screening of Multidrug Efflux Systems -- Single-Molecule Analysis of Membrane Transporter Activity by Means of a Microsystem -- Large Scale Femtoliter Droplet Array for Single Cell Efflux Assay of Bacteria -- Reconstitution and Transport Analysis of Eukaryotic Transporters in the Post-Genomic Era.
520			_aThis detailed volume utilizes our current understanding of the structural basis of multidrug recognition and multidrug efflux mechanisms to provide protocols involving these vital intrinsic membrane proteins widely distributed in bacteria. Beginning with protocols for the structural analysis of bacterial multidrug exporters, the book continues with sections on biochemical and bioengineering analysis, computational analysis, biomedical approaches, as well as advanced technologies expected to be applied to multidrug efflux transport studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Bacterial Multidrug Exporters: Methods and Protocols serves as an ideal guide to this fast moving field of study.
700	1		_aYamaguchi, Akihito _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aNishino, Kunihiko _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493974528
776	0	8	_iPrinted edition: _z9781493974535
776	0	8	_iPrinted edition: _z9781493984909
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7454-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233832 _d233832