000			03771nam a22003615i 4500
001			233834
003			ES-VaUE
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007			cr nn 008mamaa
008			180221s2018 xxu| s |||| 0|eng d
020			_a9781493975655
024	7		_a10.1007/978-1-4939-7565-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	4	_aThe Retinoblastoma Protein _cedited by Pedro G. Santiago-Cardona.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 200 páginas) _b38 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1726
505	0		_aCharacterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia -- Detection of RB1 Gene Copy Number Variations using a Multiplex Ligation-Dependent Probe Amplification Method -- A Fluorescent Quantitative Multiplex PCR Method to Detect Copy Number Changes in the RB1 Gene -- Using Methylation-specific PCR to study RB1 Promoter Hypermethylation -- Detection of Aberrant DNA Methylation Patterns in the RB1 Gene -- Detection of Retinoblastoma Protein Phosphorylation by Immunoblot Analysis -- Immunohistochemical Detection of the Retinoblastoma Protein -- Immunohistochemical Detection of Retinoblastoma Protein Phosphorylation in Human Tumor Samples -- Detection of CCND1 Locus Amplification by Fluorescence in situ Hybridization -- Detection of CCND1 Gene Copy Number Variations using Multiplex Ligation-dependent Probe Amplification and Fluorescence in situ Hybridization Methods -- Detection of p16 Promoter Hypermethylation by Methylation-specific PCR -- Immunohistochemical Detection of p16 in Clinical Samples -- Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays -- Detection of E2F-induced Transcriptional Activity Using a Dual Luciferase Reporter Assay -- Detection of HPV E6/E7 mRNA in Clinical Samples using RNA in situ Hybridization -- CRISPR/Cas9 Mediated Knockout of Rb1 in Xenopus tropicalis.
520			_aThis volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth discussions for commonly used experimental approaches to assess the status and function of components of the pRb pathway, including pRb itself, in cell lines and biological samples.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Retinoblastoma Protein aims to serve as a guide to assist molecular cancer biologists in their search for understanding of the molecular functions of this preeminent tumor suppressor.
700	1		_aSantiago-Cardona, Pedro G _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493975648
776	0	8	_iPrinted edition: _z9781493975662
776	0	8	_iPrinted edition: _z9781493985227
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7565-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233834 _d233834