000			04172nam a22003735i 4500
001			233836
003			ES-VaUE
005			20221220020524.0
007			cr nn 008mamaa
008			180524s2018 xxu| s |||| 0|eng d
020			_a9781493978656
024	7		_a10.1007/978-1-4939-7865-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMultiple Myeloma _bMethods and Protocols _cedited by Christoph Heuck, Niels Weinhold.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (X, 216 páginas) _b41 ilustraciones, 32 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1792
505	0		_aELDA qASO-PCR for High Sensitivity Detection of Tumor Cells in Bone Marrow and Peripheral Blood -- EuroFlow-Based Next-Generation Flow Cytometry for Detection of Circulating Tumor Cells and Minimal Residual Disease in Multiple Myeloma -- Cytoplasmic Immunoglobulin vs DNA Analysis by Flow Cytometry -- Deep Profiling of the Immune System of Multiple Myeloma Patients by Cytometry by Time of Flight (CyTOF) -- Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma -- Whole Exome Sequencing in Multiple Myeloma to Identify Somatic Single Nucleotide Variants and Key Translocations Involving Immunoglobulin Loci and MYC -- RNA-Sequencing from Low-Input Material in Multiple Myeloma for Application in Clinical Routine -- Protocol for M3P: A Comprehensive and Clinical Oriented Targeted Sequencing Panel for Routine Molecular Analysis in Multiple Myeloma -- Analysis of Circulating Tumor DNA -- Detection of Cross-Sample Contamination in Multiple Myeloma Samples and Sequencing Data -- Analysis of Global Gene Expression Profiles -- Genome Wide Mapping of Methylated and Hydroxyl-Methylated Cytosines using a Modified HpaII Tiny Fragment Enrichment by Ligation Mediated PCR Tagged Sequencing Protocol -- A Rapid and Robust Protocol for Reduced Representation Bisulfite Sequencing in Multiple Myeloma -- Microfluidic Production and Application of Lipid Nanoparticles for Nucleic Acid Transfection -- Microfluidic Assembly of Liposomes with Tunable Size and Co-Loading Capabilities.
520			_aThis volume provides a thorough guide of the study of multiple myeloma (MM)-from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results. The chapters in this book cover topics, such as next-generation-flow-based methods for detection of minimal residual disease and circulating tumor cells; Cytometry by Time-of-Flights (CyTOF); a droplet digital PCR approach for finding contamination in patients' samples; genome-wide investigation of the methylation and hydroxymethylation status of cytosines; and a microfluidic approach for creating nanoparticles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Multiple Myeloma: Methods and Protocols is a valuable resource for molecular scientists and laboratory supervisors working with MM samples, and any investigator conducting studies in this field.
700	1		_aHeuck, Christoph _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWeinhold, Niels _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493978649
776	0	8	_iPrinted edition: _z9781493978663
776	0	8	_iPrinted edition: _z9781493993086
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7865-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233836 _d233836