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001			233838
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008			180530s2018 xxu| s |||| 0|eng d
020			_a9781493978311
024	7		_a10.1007/978-1-4939-7831-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMitochondrial Bioenergetics _bMethods and Protocols _cedited by Carlos M. Palmeira, António J. Moreno.
250			_a2nd edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 422 páginas) _b107 ilustraciones, 59 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1782
505	0		_aOverview of Mitochondrial Bioenergetics -- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells -- High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mitochondria -- High-Throughput Analysis of Mitochondrial Oxygen Consumption -- Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices -- Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria -- Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells -- Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production -- Measurement of Proton Leak in Isolated Mitochondria -- Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy -- Mitochondrial Permeability Transition Pore and Calcium Handling -- Redox Equivalents and Mitochondrial Bioenergetics -- Mitochondrial Bioenergetics by 13 C-NMR Isotopomer Analysis -- Computational Modeling of Mitochondrial Function from a Systems Biology Perspective -- Monitoring the Mitochondrial Dynamics in Mammalian Cells -- Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria -- Plate-Based Measurement of Respiration by Isolated Mitochondria -- Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells -- The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue -- Isolation and Analysis of Mitochondrial Small RNAs from Rat Liver Tissue and HepG2 Cells -- Imaging of Mitochondrial pH Using SNARF-1 -- Relation between Mitochondrial Membrane Potential and ROS Formation -- Assessing Spatio-Temporal and Functional Organization of Mitochondrial Networks -- Measurement of Mitochondrial ROS Formation.
520			_aThis second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries.
700	1		_aPalmeira, Carlos M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMoreno, António J _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493978304
776	0	8	_iPrinted edition: _z9781493978328
776	0	8	_iPrinted edition: _z9781493992980
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7831-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233838 _d233838