000			03656nam a22003615i 4500
001			233842
003			ES-VaUE
005			20221220020525.0
007			cr nn 008mamaa
008			180601s2018 xxu| s |||| 0|eng d
020			_a9781493978601
024	7		_a10.1007/978-1-4939-7860-1 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aReporter Gene Imaging _bMethods and Protocols _cedited by Purnima Dubey.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 221 páginas) _b53 ilustraciones, 42 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1790
505	0		_aDual Modality Imaging of Promoter Activity as a Surrogate for Gene Expression and Function -- Construction of Dual Modality Optical Reporter Gene Constructs for Bioluminescent and Fluorescent Imaging -- Optical Bioluminescence Protocol for Imaging Mice -- Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase -- Reporter Based BRET Sensors for Measuring Biological Functions In Vivo -- Fluorescence Imaging of Mycobacterial Infection in Live Mice Using Fluorescent Protein-Expressing Strains -- In vivo Bacterial Imaging using Bioluminescence -- Production of GFP and Luciferase Expressing Reporter Macrophages for In Vivo Bioluminescence Imaging -- Hypoxia-induced Reporter Genes with Different Half-lives -- Visualization of Immune System Reconstitution by Bioluminescent Imaging -- Thymidine Kinase PET Reporter Gene Imaging of Cancer Cells In Vivo -- Ex vivo radiolabeling and in vivo PET imaging of T cells expressing nuclear reporter genes -- Imaging T Cell Dynamics and Function by PET using Human Nuclear Reporter Genes -- Positron Emission Tomography Imaging of Tumor Apoptosis with a Caspase-sensitive Nano-Aggregation Tracer [18F]C-SNAT -- Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes -- Synthesis, Purification, Characterization, and Imaging of Cy3-Functionalized Fluorescent Silver Nanoparticles in 2D and 3D Tumor Models.
520			_aThis volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aDubey, Purnima _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493978588
776	0	8	_iPrinted edition: _z9781493978595
776	0	8	_iPrinted edition: _z9781493993062
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7860-1 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233842 _d233842