000			03860nam a22003615i 4500
001			233843
003			ES-VaUE
005			20221220020525.0
007			cr nn 008mamaa
008			180601s2018 xxu| s |||| 0|eng d
020			_a9781493985463
024	7		_a10.1007/978-1-4939-8546-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHLA Typing _bMethods and Protocols _cedited by Sebastian Boegel.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 287 páginas) _b52 ilustraciones, 38 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1802
505	0		_aThe Past, Present, and Future of HLA Typing in Transplantation -- Role of Human Leukocyte Antigens (HLA) in Autoimmune Diseases -- The IPD Databases: Cataloguing and Understanding Allele Variants -- Allele Frequency Net Database -- High Resolution HLA-Typing by Next Generation Sequencing of Randomly Fragmented Target DNA -- High-Throughput Contiguous Full-Length Next Generation Sequencing of HLA Class I and II Genes from 96 Donors in a Single MiSeq Run -- Application of High-Throughput Next Generation Sequencing for HLA Typing on Buccal Extracted DNA -- Super High Resolution for Single Molecule-Sequence-Based Typing of Classical HLA loci using Ion Torrent PGM -- High Resolution Full-Length HLA Typing Method using Third Generation (Pac-Bio SMRT) Sequencing Technology -- Full-Length HLA Class I Genotyping with the MinION Nanopore Sequencer -- Imputation-Based HLA Typing with SNPs in GWAS Studies -- In Silico Typing of Classical and Non-Classical HLA Alleles from Standard RNA-Seq Reads -- PHLAT: Inference of High Resolution HLA Types from RNA and Whole Exome Sequencing -- Using Exome and Amplicon-Based Sequencing Data for High-Resolution HLA Typing with ATHLATES -- HLA Typing from Short-Read Sequencing Data with OptiType -- Comprehensive HLA Typing from a Current Allele Database using Next Generation Sequencing Data -- Accurate Assembly and Typing of HLA using a Graph-Guided Assembler Kourami -- AmpliSAS and AmpliHLA: Web Server Tools for MHC Typing of Non-Model Species and Human using NGS Data -- HLA Haplotype Frequency Estimation from Real-Life Data with the Hapl-o-Mat Software.
520			_aThis volume explores the rapidly evolving field of HLA typing and its use in both the laboratory setting and in silico methods. The chapters in this book discuss high-throughput methods for HLA typing; wet lab protocols; microarray data and its uses; in silico tools for the identification of HLA alleles from DNA and RNA next-generation-sequencing data, as well as HLA haplotype frequency estimation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, HLA Typing: Methods and Protocols is a valuable resource for any researcher interested in learning more about this developing field.
700	1		_aBoegel, Sebastian _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493985456
776	0	8	_iPrinted edition: _z9781493985470
776	0	8	_iPrinted edition: _z9781493993215
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8546-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233843 _d233843