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020 _a9781493985494
024 7 _a10.1007/978-1-4939-8549-4
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aImmunotoxicity Testing
_bMethods and Protocols
_cedited by Jamie C. DeWitt, Cheryl E. Rockwell, Christal C. Bowman.
250 _a2nd edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XIII, 412 páginas)
_b41 ilustraciones, 23 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1803
505 0 _aImmunotoxicology: A Brief History -- Clinical Immunotoxicology -- Investigative Immunotoxicology -- Developmental Immunotoxicity (DIT) Testing: Current Recommendations and the Future of DIT Testing -- Markers of Inflammation -- The Sheep Erythrocyte T-Dependent Antibody Response (TDAR) -- Methylated-Bovine Serum Albumin (mBSA)-Induced Delayed-Type Hypersensitivity in Mice -- Use of the LLNA:BrdU-ELISA for Skin Sensitization Hazard Assessment -- Host Resistance Assays -- Enhanced Histopathology Evaluation of Lymphoid Organs -- Tumor Challenges in Immunotoxicity Testing -- Flow Cytometry for the Immunotoxicologist -- Evaluation of Cell-Mediated Immune Function Using the Cytotoxic T Lymphocyte Assay -- Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing -- Natural Killer (NK) Cell Assays in Immunotoxicity Testing -- Dendritic Cell Assays -- Evaluating Macrophages in Immunotoxicity Testing -- Evaluating Cytokines in Immunotoxicity Testing -- Functional Assays of Hematopoietic Stem Cells in Toxicology Research -- CD4+ T Cell Differentiation and Activation -- Isolation and Identification of Innate Lymphoid Cells (ILCs) for Immunotoxicity Testing -- Evaluating Antigen-Specific IgE Using the Rat Basophil Leukemia Cell (RBL) Assay -- Challenges for Integrating Immunotoxicology into the 21st Century Toxicology Testing Paradigm.
520 _aThis fully updated volume utilizes the expertise of scientists currently engaged in immunotoxicity testing to provide the reader with lab-ready procedures and the background information needed to identify effective testing approaches. Dedicated to identifying and describing exogenous agents that can modify immune function, uncovering modes and mechanisms of action for such agents, and translating data from the laboratory and from the clinic to better predict health risks as well as benefits to those who are exposed to immunomodulatory agents, immunotoxicity testing continues to be a vital field of study, and this collection highlights both the "tried and true" methods as well as alternative protocols that have been more recently developed. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immunotoxicity Testing: Methods and Protocols, Second Edition serves as a valuable contribution to the continued evolution and the application of immunotoxicity testing.
700 1 _aDeWitt, Jamie C
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aRockwell, Cheryl E
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aBowman, Christal C
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493985487
776 0 8 _iPrinted edition:
_z9781493985500
776 0 8 _iPrinted edition:
_z9781493993222
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-8549-4
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c233845
_d233845