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001			233846
003			ES-VaUE
005			20221220020525.0
007			cr nn 008mamaa
008			140503s2014 xxu| s |||| 0|eng d
020			_a9781493907038
024	7		_a10.1007/978-1-4939-0703-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aListeria monocytogenes _bMethods and Protocols _cedited by Kieran Jordan, Edward M. Fox, Martin Wagner.
250			_a1st edition 2014
264		1	_aNew York, NY _bSpringer International Publishing _c2014
300			_a1 recurso en línea (X, 286 páginas) _b42 ilustraciones, 17 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1157
505	0		_aSampling the Production/Processing Environment for Listeria -- Traditional Methods for Isolation of Listeria monocytogenes -- Confirmation of Isolates of Listeria by Conventional and Real-Time PCR -- Serotype Assignment by Sero-Agglutination, ELISA and PCR -- Pulsed-Field Gel Electrophoresis (PFGE) Analysis of Listeria monocytogenes -- Multi-Locus Sequence Typing (MLST) of Listeria monocytogenes -- Ribotyping and Automated Ribotyping of Listeria monocytogenes -- Fluorescent Amplified Fragment Length Polymorphism (fAFLP) Analysis of Listeria monocytogenes -- High-Throughput Characterization of Listeria monocytogenes Using the OmniLog Phenotypic Microarray -- Analysis of Listeria monocytogenes Subproteomes -- The Listeria Cell Wall and Associated Carbohydrate Polymers -- Use of Bacteriophage Cell Wall Binding Proteins for Rapid Diagnostics of Listeria -- Virulence Characterization of Listeria monocytogenes -- Internalization Assays for Listeria monocytogenes -- Extraction and Analysis of Plasmid DNA from Listeria monocytogenes -- Generation of Non-Polar Deletion Mutants in Listeria monocytogenes Using the "SOEing" Method -- Mutant Construction and Integration Vector-Mediated Gene Complementation in Listeria monocytogenes -- Absolute and Relative Gene Expression in Listeria monocytogenes Using Real Time PCR -- Genome Sequencing of Listeria monocytogenes -- Using Enhanced Green Fluorescent Protein (EGFP) Promoter Fusions to Study Gene Regulation at Single Cell and Population Levels -- Control of Listeria monocytogenes in the Processing Environment by Understanding Biofilm Formation and Resistance to Sanitizers -- Vaccination Studies: Detection of a Listeria monocytogenes Specific T Cell Immune Response Using the ELISPOT Technique -- Sampling the Food Processing Environment: Taking up the Cudgel for Preventive Quality Management in Food Processing Environments.
520			_aListeria monocytogenes is still a major threat to public health. A new book in the Methods in Molecular Biology series, Listeria monocytogenes: Methods and Protocols addresses its titular pathogen with protocols and methodologies used in research to gain a better understanding of Listeria at a molecular level. The topics covered include sampling in order to isolate Listeria, methods for their identification and characterization, methods for gene manipulation, and, finally, methods for control of the organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Vital and authoritative, Listeria monocytogenes: Methods and Protocols aims to contribute to the harmonization of the methods used in the field and will therefore benefit all those interested in Listeria research.
700	1		_aJordan, Kieran _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFox, Edward M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWagner, Martin _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493907045
776	0	8	_iPrinted edition: _z9781493907021
776	0	8	_iPrinted edition: _z9781493954360
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-0703-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2014
999			_c233846 _d233846