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008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592591862
024	7		_a10.1385/1592591868 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aNeural Stem Cells _bMethods and Protocols _cedited by Tanja Zigova, Paul R. Sanberg, Juan R. Sanchez-Ramos.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (XIV, 381 páginas) _b196 ilustraciones, 43 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v198
505	0		_aIsolation and Culture of NSCs -- Neural Differentiation of Embryonic Stem Cells -- Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens -- Isolation of Stem and Precursor Cells from Fetal Tissue -- Olfactory Ensheathing Cells -- Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium -- Production of Immortalized Human Neural Crest Stem Cells -- Adult Rodent Spinal Cord Derived Neural Stem Cells -- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood -- Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers -- Characterization of NSCs in VITRO -- Analysis of Cell Generation in theTelencephalic Neuroepithelium -- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures -- Assessing the Involvement of Telomerase in Stem Cell Biology -- Detection of Telomerase Activity in Neural Cells -- In Vitro Assays for Neural Stem Cell Differentiation -- Electron Microscopy and Lac-Z Labeling -- Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells -- Fluorescence In Situ Hybridization -- RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells -- Differential Display -- Cell Labeling and Gene Misexpression by Electroporation -- Gene Therapy Using Neural Stem Cells -- Modeling Brain Pathologies Using Neural Stem Cells -- Utilization/Characterization of NSCs in Vivo -- Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model -- Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods -- Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo -- Immunocytochemical Analysis of Neuronal Differentiation -- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold -- Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS -- Optimizing Stem Cell Grafting into the CNS -- Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos -- Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells -- Routes of Stem Cell Administration in the Adult Rodent.
520			_aOver the last decade, advances in neural stem cell research have raised the hope that one day cellular therapy will not only replace missing neurons, but also replenish absent chemical signals, metabolites, enzymes, neurotransmitters, or other missing or defective components from the diseased or injured brain. In Neural Stem Cells: Methods and Protocols, internationally recognized experts from academic, clinical, and pharmaceutical laboratories describe in great detail the most frequently used cellular, molecular, and electrophysiological methods to isolate, characterize, and utilize neural stem cells. These readily reproducible techniques introduce the various sources of stem/progenitor cells, provide a wide range of conditions for their culture, and make it possible to define their properties in culture. Additional techniques are designed to help researchers identify endogenous stem cells as well as exogenous stem cells after transplantation in the brain. The protocols range from the simplest methods of isolation and characterization of neural cell properties to such very sophisticated methods as characterizing gene expression, telomerase assays, and cell cycle kinetics. Each is written by an investigator who has used the method extensively, and includes step-by-step instructions, tips on avoiding pitfalls, and invaluable notes that make all the difference to successful experimental outcomes. Comprehensive and easy-to-follow, Neural Stem Cells: Methods and Protocols provides a powerful synthesis of today's key in vitro and in vivo techniques to identify and characterize neural stem cells for research and for developing experimental therapeutics.
700	1		_aZigova, Tanja _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSanberg, Paul R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aSanchez-Ramos, Juan R _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489943743
776	0	8	_iPrinted edition: _z9781489943736
776	0	8	_iPrinted edition: _z9780896039643
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592591868 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c233847 _d233847