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007			cr nn 008mamaa
008			150818s2015 xxu| s |||| 0|eng d
020			_a9781493929177
024	7		_a10.1007/978-1-4939-2917-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aVEGF Signaling _bMethods and Protocols _cedited by Lorna Fiedler.
250			_a1st edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (X, 225 páginas) _b36 ilustraciones, 23 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1332
505	0		_aVEGF Splicing and the Role of VEGF Splice Variants: From Physiological-Pathological Conditions to Specific Pre-mRNA Splicing -- Detection and Quantification of VEGF Isoforms by ELISA -- Quantitation of Circulating Neuropilin-1 in Human, Monkey, Mouse and Rat Sera by ELISA -- Detection and Quantification of Vascular Endothelial Growth Factor Receptor Tyrosine Kinases in Primary Human Endothelial Cells -- Induction of VEGF Secretion in Cardiomyocytes by Mechanical Stretch -- Chromatin Immunoprecipitation Assay: Examining the Interaction of NFkB with the VEGF Promoter -- An Overview of VEGF-Mediated Signal Transduction -- Identification of Receptor Tyrosine Kinase Inhibitors using Cell Surface Biotinylation and Affinity Isolation -- Analysis of VEGF-Mediated ERK5 Activity in Endothelial Cells -- In Vitro Angiogenesis Assays -- Chemotactic Migration of Endothelial Cells Towards VEGF-A165 -- Vasculogenesis and Angiogenesis in VEGF Receptor-1 Deficient Mice -- The Embryonic Mouse Hindbrain and Postnatal Retina as In Vivo Models to Study Angiogenesis -- VEGF Gene Transfer to the Utero-Placental Circulation of Pregnant Guinea Pigs to Enhance Fetal Growth -- VEGF Gene Transfer to the Utero-placental Circulation of Pregnant Sheep to Enhance Fetal Growth -- Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System.
520			_aThis volume provides a collection of protocols for studying and manipulating VEGF signaling pathways in vitro and in vivo, and in particular, aims to present a range of both firmly established and newly emerging technologies, including those that are amenable to aiding in drug discovery or pre-clinical investigations. Each of the three sections begins with an introductory overview followed by supporting relevant methods. A major advantage of this book is that each chapter not only contains methodological detail rarely seen in other literature, but also contains a section on key notes and troubleshooting advice. Part I focuses on quantification of specific VEGF and VEGF receptor isoforms, beginning with a summary on VEGF splice variants, their function, and their regulation. Part II relates to the study of VEGF dependent signaling in vitro, starting with a comprehensive overview of VEGF-mediated signal transduction in the cardiovascular system that highlights recent discoveries in the field. Part III first details genetic mouse models, then discusses techniques for studying physiological angiogenesis in the developing mouse embryo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, VEFG Signaling: Methods and Protocols is a useful tool for researching and understanding the basic biology of VEGF signaling, and translating it into the clinic.
700	1		_aFiedler, Lorna _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493929160
776	0	8	_iPrinted edition: _z9781493929184
776	0	8	_iPrinted edition: _z9781493943708
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2917-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c233849 _d233849