000			04950nam a22003735i 4500
001			233852
003			ES-VaUE
005			20221220020525.0
007			cr nn 008mamaa
008			100301s2004 xxu| s |||| 0|eng d
020			_a9781592597826
024	7		_a10.1385/1592597823 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlatelets and Megakaryocytes _bVolume 1: Functional Assays _cedited by Jonathan M. Gibbins, Martyn P. Mahaut-Smith.
250			_a1st edition 2004
264		1	_aTotowa, NJ _bHumana Press _c2004
300			_a1 recurso en línea (XIX, 385 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v272
505	0		_aPlatelet Functional Assays -- Effects of Anticoagulants Used During Blood Collection on Human Platelet Function -- Preparation of Washed Platelet Suspensions From Human and Rodent Blood -- Platelet Counting -- Electron Microscopy Methods for Studying Platelet Structure and Function -- Platelet Aggregation -- Platelet Aggregation in Whole Blood -- Secretion From Dense Granules -- Platelet Dense-Granule Secretion -- Analysis of the Releasable Nucleotides of Platelets -- Studies of Secretion Using Permeabilized Platelets -- Measurement of Platelet Arachidonic Acid Metabolism -- Measurement of the Platelet Procoagulant Response -- Platelet Adhesion Assays Performed Under Static Conditions -- Platelet Adhesion Assays Under Flow Using Matrix Protein-Coupled Adhesion Columns -- Techniques to Examine Platelet Adhesive Interactions Under Flow -- In Vivo Models of Platelet Function and Thrombosis -- Adhesion Between Platelets and Leukocytes or Endothelial Cells -- In Vitro Measurement of High-Shear Platelet Adhesion and Aggregation by the PFA-100® -- Flow-Cytometric Analysis of Platelet-Membrane Glycoprotein Expression and Platelet Activation -- Flow-Cytometric Analysis of Mouse Platelet Function -- Platelet Microparticles -- Megakaryocyte Functional Assays -- Isolation of Primary Megakaryocytes and Studies of Proplatelet Formation -- Isolation and Culture of Megakaryocyte Precursors -- Assays of Megakaryocyte Development -- Assays of Megakaryocyte Development -- Thrombopoietin Bioassay -- Culture of Megakaryocytic Cell Lines.
520			_aPlatelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 1: Functional Assays, leading expert researchers-many responsible for seminal discoveries in the field-describe basic and advanced techniques for analyzing platelet and megakaryocyte function in step-by-step detail. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. Techniques presented for megakaryocyte function research include those used to study the development of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques offers a state-of-the-art survey of our understanding of these cells, with emphasis on the role of cell signaling in their regulation and function, as well as the use of molecular and post-genomic techniques for their study. Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.
700	1		_aGibbins, Jonathan M _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aMahaut-Smith, Martyn P _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617373565
776	0	8	_iPrinted edition: _z9781588291011
776	0	8	_iPrinted edition: _z9781489942197
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597823 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2004
999			_c233852 _d233852