000			03743nam a22003615i 4500
001			233859
003			ES-VaUE
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007			cr nn 008mamaa
008			170726s2017 xxu| s |||| 0|eng d
020			_a9781493971756
024	7		_a10.1007/978-1-4939-7175-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicroRNA Technologies _cedited by Min Jeong Kye.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XII, 216 páginas) _b20 ilustraciones, 10 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v128
505	0		_aProtocol for miRNA In Situ Hybridization on Mouse Spinal Cord -- Protocol for High Content Screening for the Impact of Overexpressed MicroRNAs on Primary Motor Neurons -- Quantification of Dicer Activity in Mammalian Cell Lysates Using a Non-Radioactive Fluorescence Method -- Analysis of MicroRNAs and their Potential Targets in Human Embryonic Stem Cell-Derived Neurons Treated with the Anesthetic Protocol -- MicroRNA Expression Profiling by PCR Array in 2D and 3D Differentiated Neural Culture Systems and Target Validation -- Study of miRNA Function in the Developing Axons of Mouse Cortical Neurons: Use of Compartmentalized Microfluidic Chambers and In Utero Electroporation -- Functional Analysis of Cortical Neuron Migration Using miRNA Silencing -- Continuous Delivery of Oligonucleotides into the Brain -- Isolating and Screening Subcellular miRNAs in Neuron -- Experimental Methods for Functional Studies in MicroRNAs in Animal Models of Psychiatric Disorders -- Isolation and Quantitative Analysis of Axonal Small Noncoding RNAs -- In Ovo Electroporation of miRNA Plasmids to Silence genes in a Temporally and Spatially Controlled Manner -- Three Dimensional Cell Culture of Human Neural Stem Cells using Polysaccharide-Based Hydrogels and Subsequent Bioanalyses -- Measuring miRNA Mediated Translational Regulation with Live Cell Imaging.
520			_aThis volume examines different approaches toward understanding the function of microRNA (miRNA) and the tools used to regulate microRNA expression in nervous systems. MicroRNA Technologies also explores newly established techniques to study the role of miRNAs in the nervous system. The chapters in this book cover topics such as: high content screening for looking at the impact of overexpressed miRNAs on primary motor neurons; miRNA expression profiling by PCR array in 2D and 3D neural culture systems; analysis of cortical neuron migration using miRNA silencing; isolating and screening subcellular miRNAs in neurons; and measuring miRNA mediated translational regulation with live cell imaging. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals. Cutting-edge and practical, MicroRNA Technologies is a valuable resource for students and researchers who are interested about microRNAs in nervous systems.
700	1		_aKye, Min Jeong _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493971732
776	0	8	_iPrinted edition: _z9781493971749
776	0	8	_iPrinted edition: _z9781493984077
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7175-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233859 _d233859