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008			150808s2016 xxu| s |||| 0|eng d
020			_a9781493929573
024	7		_a10.1007/978-1-4939-2957-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCell Cycle Oscillators _bMethods and Protocols _cedited by Amanda S. Coutts, Louise Weston.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XII, 352 páginas) _b80 ilustraciones, 60 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1342
505	0		_aCell Cycle Control: A System of Interlinking Oscillators -- Model Organisms for Studying the Cell Cycle -- Role of Computational Modelling in Understanding Cell Cycle Oscillators -- E2F Transcription Factors Control the Roller Coaster Ride of Cell Cycle Gene Expression -- Cell Synchronization of Mouse Embryonic Fibroblasts -- Cell Cycle Synchronisation in Xenopus Egg Extracts -- Elutriation for Cell Cycle Synchronization in Fission Yeast -- Spatiotemporal Investigation of Phosphorylation Events during Cell Cycle Progression -- Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence -- Building a Synthetic Transcriptional Oscillator -- Time Series Simulations and Bifurcation Analyses of a Model of Mitotic Oscillations in Xenopus Cell-free Extracts -- A Computational Method for Identifying Yeast Cell Cycle Transcription Factors -- Measuring Activity and Specificity of Protein Phosphatases -- Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an in vitro Cdk1 Targeting Assay to Study the Regulation of the g-Tubulin Complex -- Cell Cycle Synchronisation Using a Microfluidic Synchroniser for Fission Yeast Cells -- Detection of Protein-Protein Interactions in Tobacco BY-2 cells Using BimolecularFluorescence Complementation -- Tracking the Cyclin B1-GFP Sensor to Profile the Pattern of Mitosis Versus Mitotic Bypass -- Measuring APC/C Dependent Ubiquitylation in vitro -- Using the Fly-FUCCI System for the Live Analysis of Cell Cycle Dynamics in Cultured Drosophila cells -- Imaging Cell Cycle Phases and Transitions of Living Cells From Yeast to Woman -- Measurement of Cdk1/cyclin B Kinase Activity by Specific Antibodies and Western Blotting -- The use of SNAP Labeling to Study Cell Cycle Oscillatory Proteins.
520			_aThis volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.
700	1		_aCoutts, Amanda S _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aWeston, Louise _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493929566
776	0	8	_iPrinted edition: _z9781493929580
776	0	8	_iPrinted edition: _z9781493949588
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2957-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c233860 _d233860