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001			233865
003			ES-VaUE
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007			cr nn 008mamaa
008			150306s2015 xxu| s |||| 0|eng d
020			_a9781493924479
024	7		_a10.1007/978-1-4939-2447-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAffinity Chromatography _bMethods and Protocols _cedited by Senta Reichelt.
250			_a3rd edition 2015
264		1	_aNew York, NY _bSpringer International Publishing _c2015
300			_a1 recurso en línea (XIII, 341 páginas) _b78 ilustraciones, 37 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1286
505	0		_aAffinity Chromatography: A Historical -- Protein Purification by Aminosquarylium Cyanine-Affinity Chromatography -- One Step Purification of Phosphinothricin Acetyltransferase using Reactive Dye Affinity Chromatography -- Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes -- Specific Recognition of Supercoiled Plasmid DNA by Affinity Chromatography using a Synthetic Aromatic Ligand -- Cell Affinity Separations on Microfluidic Devices -- Aptamer-modified :Magnetic Beads in Affinity Separation of Proteins -- The Strep-tag System for One-step Purification and High Affinity Detection or Capturing of Proteins -- Robotic High-throughput Purification of Affinity-tagged Recombinant Proteins -- Macroporous Silica Particles Derivatized for Enhanced Lectin Affinity Enrichment of Glycoproteins -- Immobilized Magnetic Beads based Multi-Target Affinity Selection Coupled with HPLC-MS for Screening Active Compounds from Traditional Chinese Medicine and Natural Products -- Mixed-bed Affinity Chromatography: Principles And Methods -- Preparation and characterization of fluorophenylboronic acid-functionalized Affinity Monolithic Columns for the Selective Enrichment of Cis-diol-containing Biomolecules -- Introduction to Macroporous Cryogels -- Cryogels with Affinity Ligands as Tools in Protein Purification -- Direct Capture of His6-tagged Proteins Using Megaporous Cryogels Developed for Metal-ion Affinity Chromatography -- Ni(II) Chelated IDA Functionalized Poly(HEMA-GMA) Cryogels for Urease Adsorption -- A Novel Chromatographic Media: Histidine Containing Composite Cryogels for HIgG Separation from Human Serum --  Molecularly Imprinted Cryogels for Human Serum Albumin Depletion -- Interpenetrating Polymer Network Composite Cryogels with Tailored Porous Morphology and Sorption Properties -- Analysis of Drug-Protein Interactions by High-Performance Affinity Chromatography: Interactions of Sulfonylurea Drugs with Normal and Glycated Human Serum Albumin -- Accurate protein-peptide Titration Experiments by Nuclear Magnetic Resonance using Low-volume Samples -- Characterization of the Binding Strengths between Boronic Acids and Cis-Diol-containing Biomolecules by Affinity Capillary Electrophoresis -- Determination of the Kinetic Rate Constant of Cyclodextrin Supramolecular Systems by High Performance Affinity Chromatography -- Molecular Modeling of the Affinity Chromatography of Monoclonal Antibodies.
520			_aThe aim of this edition is to introduce the beginner to the basics of affinity chromatography and provide practical knowledge for the development of affinity separation protocols. Affinity Chromatography: Methods and Protocols, Third Edition guides readers through new state of the art protocols,  molecular modelling,  and the study of ligand-target interactions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.     Authoritative and easily accessible, Affinity Chromatography: Methods and Protocols, Third Edition is  designed as a useful resource for those interested in the rapid and quantitative isolation of biomolecules with high purity.
700	1		_aReichelt, Senta _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493924462
776	0	8	_iPrinted edition: _z9781493924486
776	0	8	_iPrinted edition: _z9781493955848
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-2447-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2015
999			_c233865 _d233865