000			03999nam a22003615i 4500
001			233868
003			ES-VaUE
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007			cr nn 008mamaa
008			130517s2013 xxu| s |||| 0|eng d
020			_a9781627034418
024	7		_a10.1007/978-1-62703-441-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aCyclic Nucleotide Signaling in Plants _bMethods and Protocols _cedited by Chris Gehring.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 273 páginas) _b41 ilustraciones, 25 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1016
505	0		_aMolecular Methods for the Study of Signal Transduction in Plants -- Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains -- Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS -- Determination of ADP-ribosyl cyclase activity, cyclic ADP-ribose and Nicotinamide adenine dinucleotide phosphate in Tissue Extracts -- In vivo imaging of cGMP in plants -- Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses -- Non-invasive Microelectrode Ion Flux Estimation Technique (MIFE) for the study of the regulation of root membrane transport by cyclic nucleotides -- Calcium imaging of the cyclic nucleotide response -- Identification and Quantification of Signal Molecule-dependent Protein Phosphorylation -- Comparative Gel-based (Phospho)proteomics in Response to Signaling Molecules -- An Affinity Pull-down Approach to Identify the Plant Cyclic Nucleotide Interactome -- Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activit -- Computational Identification of Candidate Nucleotide Cyclases in Higher Plants -- Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions -- Inferring biological functions of guanylyl cyclases with computational methods -- Identification and Characterization of Cyclic Nucleotide Phosphodiesterases -- Detection of reactive oxygen species down-stream of cyclic nucleotide signals in plants -- Measurement of nitric oxide in plant tissue using difluorofluorescein and oxyhaemoglobin -- Infra-red Gas Analysis (IRGA) Technique for the Study of the Regulation of Photosynthetic Responses.
520			_aOver the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.     Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.
700	1		_aGehring, Chris _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627034425
776	0	8	_iPrinted edition: _z9781627034401
776	0	8	_iPrinted edition: _z9781493962914
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-441-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c233868 _d233868