000			04854nam a22003615i 4500
001			233869
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007			cr nn 008mamaa
008			100301s2009 xxu| s |||| 0|eng d
020			_a9781603274692
024	7		_a10.1007/978-1-60327-469-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aWnt Signaling _bVolume 2, Pathway Models _cedited by Elizabeth Vincan.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (XVI, 494 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v469
505	0		_aEvolution of the Wnt Pathways -- Dictyostelium -- Dictyostelium Development: A Prototypic Wnt Pathway? -- Monitoring Patterns of Gene Expression in Dictyostelium by ?-galacotsidase Staining -- Use of the Dictyostelium Stalk Cell Assay to Monitor GSK-3 Regulation -- Cnidarians -- Wnt Signaling in Cnidarians -- Detecting Expression Patterns of Wnt Pathway Components in Nematostella vectensis Embryos -- Detection of Expression Patterns in Hydra Pattern Formation -- C. Elegans -- Analysis of Wnt Signaling During Caenorhabditis elegans Postembryonic Development -- Wnt Signaling During Caenorhabditis elegans Embryonic Development -- Drosophila -- Function of the Wingless Signaling Pathway in Drosophila -- Visualization of PCP Defects in the Eye and Wing of Drosophila melanogaster -- Wingless Signaling in Drosophila Eye Development -- High-Throughput RNAi Screen in Drosophila -- Sea Urchin -- Wnt Signaling in the Early Sea Urchin Embryo -- Detecting Expression Patterns of Wnt Pathway Components in Sea Urchin Embryos -- Functional Analysis of Wnt Signaling in the Early Sea Urchin Embryo Using mRNA Microinjection -- Zebrafish -- Wnt Signaling Mediates Diverse Developmental Processes in Zebrafish -- Determination of mRNA and Protein Expression Patterns in Zebrafish -- Manipulation of Gene Expression During Zebrafish Embryonic Development Using Transient Approaches -- Neural Patterning and CNS Functions of Wnt in Zebrafish -- Xenopus -- Studying Wnt Signaling in Xenopus -- Analysis of Gene Expression in Xenopus Embryos -- Detection of Nuclear ?-catenin in Xenopus Embryos -- Transgenic Reporter Tools Tracing Endogenous Canonical Wnt Signaling in Xenopus -- Gain-of-Function and Loss-of-Function Strategies in Xenopus -- How The Mother Can Help: Studying Maternal Wnt Signaling by Anti-sense-mediated Depletion of Maternal mRNAs and the Host Transfer Technique -- Inducible Gene Expression in Transient Transgenic Xenopus Embryos -- Wnt-Frizzled Interactions in Xenopus -- Dorsal Axis Duplication as a Functional Readout for Wnt Activity -- Regulation of Convergent Extension by Non-canonical Wnt Signaling in the Xenopus Embryo -- Frizzled-7-dependent Tissue Separation in the Xenopus Gastrula.
520			_aSince their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study. Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In Volume 2, Pathway Models, the diverse vertebrate and invertebrate models that have shaped the Wnt signaling field are described, presenting an overview of the unique properties of each organism, like asymmetric cell division in C. elegans and epithelial morphogenesis in Dictyostelium, with respect to studying Wnt/FZD function. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips. Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource. .
700	1		_aVincan, Elizabeth _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781603279888
776	0	8	_iPrinted edition: _z9781617378997
776	0	8	_iPrinted edition: _z9781603274685
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60327-469-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c233869 _d233869