000			03916nam a22003495i 4500
001			233871
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007			cr nn 008mamaa
008			100301s2005 xxu| s |||| 0|eng d
020			_a9781592598618
024	7		_a10.1385/1592598617 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aHuman Cell Culture Protocols _cedited by Joanna Picot.
250			_a2nd edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (XII, 360 páginas) _b120 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Medicine _x1940-6037 _v107
505	0		_aEstablishment and Maintenance of Normal Human Keratinocyte Cultures -- Cultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol Esters -- Isolation and Culture of Human Osteoblasts -- Human Osteoclast Culture From Peripheral Blood Monocytes -- Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation -- Human Myoblasts and Muscle-Derived SP Cells -- Cell Cultures of Autopsy-Derived Fibroblasts -- Primary Culture and Differentiation of Human Adipocyte Precursor Cells -- Human Mononuclear Phagocytes in Tissue Culture -- Purification of Peripheral Blood Natural Killer Cells -- Human Fetal Brain Cell Culture -- Culturing Human Schwann Cells -- Well-Differentiated Human Airway Epithelial Cell Cultures -- Isolation and Culture of Human Alveolar Epithelial Cells -- A New Approach to Primary Culture of Human Gastric Epithelium -- Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a Noninjurious Approach -- Isolation and Culture of Human Hepatocytes -- Glomerular Epithelial and Mesangial Cell Culture and Characterization -- Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules -- Culture of Parathyroid Cells -- Long-Term Culture and Maintenance of Human Islets of Langerhans in Memphis Serum-Free Media -- Primary Culture of Human Antral Endocrine and Epithelial Cells -- Conjunctiva Organ and Cell Culture -- Establishment, Maintenance, and Transfection of In Vitro Cultures of Human Retinal Pigment Epithelium.
520			_aIn this second edition of a popular and widely acclaimed collection of laboratory methods, a panel of leading authorities have thoroughly brought up-to-date and optimized its cell culture techniques for a broad range of human cell types relevant to human disease. Each technique can be used to investigate a wide spectrum of important processes, ranging from the pathogenesis of disease, to the study of metabolic processes, to control of proliferation and differentiation. New to this edition are chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Wide-ranging and highly practical, Human Cell Culture, Second Edition, provides novice and experienced researchers alike with a detailed, step-by-step guide to successful culture human cells today.
700	1		_aPicot, Joanna _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617374319
776	0	8	_iPrinted edition: _z9781588292223
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598617 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2005
999			_c233871 _d233871