000			03453nam a22003615i 4500
001			233872
003			ES-VaUE
005			20221220020527.0
007			cr nn 008mamaa
008			110414s2009 xxu| s |||| 0|eng d
020			_a9781597454940
024	7		_a10.1007/978-1-59745-494-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTransgenic Maize _bMethods and Protocols _cedited by M. Paul Scott.
250			_a1st edition 2009
264		1	_aTotowa, NJ _bHumana Press _c2009
300			_a1 recurso en línea (IX, 188 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v526
505	0		_aDesign of Gene Constructs for Transgenic Maize -- Transformation Methods -- Transient Expression of GFP in Immature Seed Tissues -- Biolistic Gun-Mediated Maize Genetic Transformation -- Agrobacterium-Mediated Maize Transformation: Immature Embryos Versus Callus -- Whiskers-Mediated Maize Transformation -- Transgenic Maize in Research -- Methods for Generation and Analysis of Fluorescent Protein-Tagged Maize Lines -- Use of Transgene-Induced RNAi to Regulate Endogenous Gene Expression -- Plasmid Rescue: Recovery of Flanking Genomic Sequences from Transgenic Transposon Insertion Sites -- Analysis of Transgenic Plants -- Nonradioactive Genomic DNA Blots for Detection of Low Abundant Sequences in Transgenic Maize -- Tissue-Print Immunodetection of Transgene Products in Endosperm for High-Throughput Screening of Seeds -- Determination of Transgene Copy Number by Real-Time Quantitative PCR -- Breeding with Transgenes -- Herbicide Resistance Screening Assay -- Characterizing Transgene Inheritance -- Backcross Breeding.
520			_aAs increasing global population and continuing economic development ensure the need for further production and cultivation of maize, the necessity of the application of transgenic technology to this model species and crop plant grows steadily. In Transgenic Maize: Methods and Protocols, experts in the various disciplines of the field contribute readily reproducible protocols covering such topics as transformation methods, transgenic maize in research, the analysis of transgenic plants, and breeding with transgenes. Written in the highly successful Methods in Molecular Biology™ series format, the chapters present brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Transgenic Maize: Methods and Protocols will greatly aid researchers wishing to use these procedures in their own laboratories, and will provide readers with a better understanding of the experiments being performed by cooperators or fee-for-service labs.
700	1		_aScott, M. Paul _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617379352
776	0	8	_iPrinted edition: _z9781603278355
776	0	8	_iPrinted edition: _z9781934115497
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-494-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2009
999			_c233872 _d233872