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020 _a9781627036191
024 7 _a10.1007/978-1-62703-619-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPheromone Signaling
_bMethods and Protocols
_cedited by Kazushige Touhara.
250 _a1st edition 2013
264 1 _aTotowa, NJ
_bHumana Press
_c2013
300 _a1 recurso en línea (XV, 399 páginas)
_b74 ilustraciones, 30 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1068
505 0 _aFemale Moth Pheromones -- Pheromones in the Fruit Fly -- Analysis of Volatile Mouse Pheromones by Gas Chromatography Mass Spectrometry -- Murine Non-Volatile Pheromones: Isolation of Exocrine-Gland Secreting Peptide 1 -- Chemical Analysis of Aquatic Pheromones in Fish -- Analysis of Ascarosides from Caenorhabditis elegans Using Mass Spectrometry and NMR Spectroscopy -- Identification of Chemosensory Receptor Genes from Vertebrate Genomes -- Functional Assays for Insect Olfactory Receptors in Xenopus Oocytes -- A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors -- Genetic Manipulation to Analyze Pheromone Responses: Knockouts of Multiple Receptor Genes -- Electro-Antennogram and Single Sensillum Recording in Insect Antennae -- Calcium Imaging of Pheromone Responses in the Insect Antennal Lobe -- Live Cell Calcium Imaging of Dissociated Vomeronasal Neurons -- Whole Mount Imaging of Responses in Mouse Vomeronasal Neurons -- Calcium Imaging of Vomeronasal Organ Response Using Slice Preparations from Transgenic Mice Expressing G-CaMP2 -- The Electrovomeronasogram: Field Potential Recordings in the Mouse Vomeronasal Organ -- Electrical Recordings from the Accessory Olfactory Bulb in VNO-AOB Ex Vivo Preparations -- Pheromone-Induced Expression of Immediate Early Genes in the Mouse Vomeronasal Sensory System -- Insect Pheromone Behavior: Fruit Fly -- Quantitative Assessment of Pheromone-Induced Dauer Formation in Caenorhabditis elegans -- Acute Behavioral Responses to Pheromones in C. elegans (Adult Behaviors: Attraction, Repulsion) -- Behavioral Analysis of Pheromones in Fish -- Analysis of Male Aggressive and Sexual Behavior in Mice -- Assessment of Urinary Pheromone Discrimination, Partner Preference, and Mating Behaviors in Female Mice -- Assessing Postpartum Maternal Care, Alloparental Behavior, and Infanticide in Mice: With Notes on Chemosensory Influences -- Testing Smell When It is Really Vital: Behavioral Assays of Social Odors in the Neonatal Mouse -- An Assay for Human Chemosignals.
520 _aProviding experimental methods and protocols for performing pheromone research in a variety of organisms ranging from invertebrates to vertebrates, Pheromone Signaling: Methods and Protocols covers a wide spectrum of experimental approaches necessary for handling pheromone molecules, measuring receptor response and neural activation, and analyzing behavioral output. A great deal of progress has been made in understanding the molecular mechanisms underlying pheromone action, largely due to the discovery of receptor genes, the advancement of imaging techniques, and key multi-disciplinary approaches including aspects of organic chemistry, biochemistry, molecular biology, electrophysiology, and behavioral science. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.   Authoritative and practical, Pheromone Signaling: Methods and Protocols details the key methodologies utilized in laboratories all over the world, making them accessible to those who want to begin investigation in the area of pheromone research.
700 1 _aTouhara, Kazushige
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781627036207
776 0 8 _iPrinted edition:
_z9781627036184
776 0 8 _iPrinted edition:
_z9781493960125
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-619-1
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2013
999 _c233873
_d233873