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001			233881
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008			100301s2005 xxu| s |||| 0|eng d
020			_a9781592599486
024	7		_a10.1007/978-1-59259-948-6 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aChemical Genomics _bReviews and Protocols _cedited by Edward D. Zanders.
250			_a1st edition 2005
264		1	_aTotowa, NJ _bHumana Press _c2005
300			_a1 recurso en línea (XII, 280 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v310
505	0		_aReviews -- to Chemical Genomics -- Chemistry for Chemical Genomics -- Computer-Aided Design of Small Molecules for Chemical Genomics -- Protocols -- Design, Synthesis, and Screening of Biomimetic Ligands for Affinity Chromatography -- The Role and Application of In Silico Docking in Chemical Genomics Research -- Synthesis of Complex Carbohydrates and Glyconjugates -- High-Throughput Cloning for Proteomics Research -- Screening for the Expression of Soluble Recombinant Protein in Escherichia coli -- High-Throughput Purification of Hexahistidine-Tagged Proteins Expressed in E. coli -- The Wheat Germ Cell-Free Expression System -- Advances in Genome-Wide Protein Expression Using the Wheat Germ Cell-Free System -- Production of Proteins for NMR Studies Using the Wheat Germ Cell-Free System -- Adenoviral Expression of Reporter Proteins for High-Throughput Cell-Based Screening -- Fabrication of Protein Function Microarrays for Systems-Oriented Proteomic Analysis -- Peptide and Small-Molecule Microarrays -- Peptide Mass Fingerprinting -- A Practical Protocol for Carbohydrate Microarrays -- Development of a Yeast Two-Hybrid Screen for Selection of Human Ras-Raf Protein Interaction Inhibitors.
520			_aChemical genomics is an exciting new field that promises to transform biological chemistry into a high-throughput industrialized process, much in the same way that genomics has transformed molecular biology. In Chemical Genomics: Reviews and Protocols, leading edge researchers introduce the uninitiated into the various "flavors" of chemical genomics and describe a series of readily reproducible techniques for applications in organic chemistry, protein chemistry, and computer science, with emphasis on the high-throughput approaches that are the essence of chemical genomics. The techniques range from the classic design of small molecules as affinity ligands for specific protein families, to in silico docking for computational approaches, to protein-small molecule interactions, to producing highly diverse collections of proteins, carbohydrates, and small molecules for use in arrays containing large numbers of these molecules. There are also methods for the production of proteins and carbohydrates using different expression systems, for analyzing proteins using mass spectrometry, and for arraying proteins and carbohydrates on solid supports. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Authoritative and timely, Chemical Genomics: Reviews and Protocols offers researchers an accessible introduction by pioneers to the key areas and methods of chemical genomics, as well as its use in the discovery and evaluation of novel drug targets.
700	1		_aZanders, Edward D _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617375415
776	0	8	_iPrinted edition: _z9781588293992
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59259-948-6 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2005
999			_c233881 _d233881