000			04007nam a22003615i 4500
001			233884
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007			cr nn 008mamaa
008			151103s2016 xxu| s |||| 0|eng d
020			_a9781493931484
024	7		_a10.1007/978-1-4939-3148-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRNA Imaging _bMethods and Protocols _cedited by Zdravka Medarova.
250			_a1st edition 2016
264		1	_aNew York, NY _bSpringer International Publishing _c2016
300			_a1 recurso en línea (XI, 218 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1372
505	0		_aImaging Functional Nucleic Acid Delivery to Skin -- In Vivo Magnetic Resonance Imaging of Small Interfering RNA Nanodelivery to Pancreatic Islets -- Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy -- Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer -- Analyses of Tumor Burden In Vivo and Metastasis Ex Vivo Using Luciferase-Expressing Cancer Cells in an Orthotopic Mouse Model of Neuroblastoma -- Indium-Labeling of siRNA for Small Animal SPECT Imaging -- Imaging of Electrotransferred siRNA -- Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents -- siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo -- Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells -- Molecular Beacon-Based MicroRNA Imaging During Neurogenesis -- Hypoxia-Responsive Copolymer for siRNA Delivery -- Controlling RNA Expression in Cancer Using Iron Oxide Nanoparticles Detectable by MRI and In Vivo Optical Imaging -- Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures -- Positive Bioluminescence Monitoring of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES -- MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery.
520			_aThis detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of imaging methodologies, and their interpretation. An essential element in the development and optimization of these therapies is the ability to measure the bioavailability and functionality of the RNA/oligonucleotide molecule after administration into the body. Noninvasive imaging provides the necessary set of tools to accomplish this in authentic physiologic environments and across time. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, RNA Imaging: Methods and Protocols serves physicians, scientists, and graduate students who are either new to the field of RNA-based imaging and its associated therapeutic applications or who wish to be apprised of recent advances in the state of the art.
700	1		_aMedarova, Zdravka _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493931477
776	0	8	_iPrinted edition: _z9781493931491
776	0	8	_iPrinted edition: _z9781493949748
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3148-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2016
999			_c233884 _d233884