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008			130820s2013 xxu| s |||| 0|eng d
020			_a9781627035774
024	7		_a10.1007/978-1-62703-577-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMetabolomics Tools for Natural Product Discovery _bMethods and Protocols _cedited by Ute Roessner, Daniel Anthony Dias.
250			_a1st edition 2013
264		1	_aTotowa, NJ _bHumana Press _c2013
300			_a1 recurso en línea (X, 311 páginas) _b83 ilustraciones, 33 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1055
505	0		_aBryophytes: Liverworts, Mosses and Hornworts - Extraction and Isolation Procedures -- Plant Tissue Extraction for Metabolomics -- Detection of Polar Metabolites through the use of Gas Chromatography - Mass Spectrometry -- A Robust GC-MS Method for the Quantitation of Fatty Acids in Biological Systems -- A Workflow from Untargeted LC-MS Profiling to Targeted Natural Product Isolation -- Lipidomics -Extraction Protocols for Biological Matrices -- Metabolite Analysis of Biological Fluids and Tissues by Proton Nuclear Magnetic Resonance Spectroscopy -- NMR Spectroscopy: Structure Elucidation of Cycloelatenene A: A Natural Product Case Study -- NMR-Based Metabolomics: A Probe to Utilize Biodiversity -- Extraction Protocol for Non-Targeted NMR and LC-MS Metabolomics-Based Analysis of Hard Coral and their Algal Symbionts -- Determination of Absolute Configuration Using Single Crystal X-ray Diffraction -- Natural Product Chemistry in Action:  The Synthesis of Melatonin Metabolites K1 and K2 -- Discovery, Biosynthesis, and Rational Engineering of Novel Enterocin and Wailupemycin Polyketide Analogues -- Bioassays for Anticancer Activities -- Screening for Anti-Diabetic Activities -- Screening for Antibacterial, Antifungal and Anti-Quorum Sensing Activity -- Metabolomics and Dereplication Strategies in Natural Products -- Bridging the Gap: Basic Metabolomics Methods for Natural Product Chemistry -- Strategies in Biomarker Discovery. Peak Annotation by MS and Targeted LC-MS Micro-Fractionation for de novo Structure Identification by Micro-NMR -- Statistical Analysis of Metabolomics Data.
520			_aClassical natural product chemistry is transitioning to modern day metabolomics as a result of the advent of comprehensive analytical platforms and sensitive analytical instrumentation. Therefore, it is worthwhile to summarize recent developments with current analytical platforms and highlight how metabolomics is being integrated into this classical field to dereplicate and profile natural product extracts. Metabolomics Tools for Natural Product Discoveries: Methods and Protocols aims to unite diverse and recently developed methodologies and protocols in order to identify bioactive secondary metabolites for the purpose of drug discovery. Some topics covered in this volume include applications for the extraction of selected natural products from less common sources such as bryophytes and hard corals, various biological assays, comprehensive applications and strategies for GC-MS, LC-MS, and NMR, as well as protocols and strategies for the structure elucidation of isolated natural products.  Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and easily accessible Metabolomics Tools for Natural Product Discoveries: Methods and Protocols seeks to serve both professionals and research students with its well-honed methodologies for natural product isolation, biomarker discovery, dereplication, biological assays, and comprehensive metabolomic platforms available for high-throughput analyses.
700	1		_aRoessner, Ute _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aDias, Daniel Anthony _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781627035781
776	0	8	_iPrinted edition: _z9781627035767
776	0	8	_iPrinted edition: _z9781493963010
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-62703-577-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2013
999			_c233894 _d233894