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020 _a9781493970032
024 7 _a10.1007/978-1-4939-7003-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlant Genomics
_bMethods and Protocols
_cedited by Wolfgang Busch.
250 _a1st edition 2017
264 1 _aNew York, NY
_bSpringer International Publishing
_c2017
300 _a1 recurso en línea (XI, 317 páginas)
_b74 ilustraciones, 60 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1610
505 0 _aCRISPR/Cas-mediated in planta Gene Targeting -- User Guide for the LORE1 Insertion Mutant Resource -- Enabling Reverse Genetics in Medicago truncatula Using High Throughput Sequencing for Tnt1 Flanking Sequence Recovery -- Generation of Doubled Haploid Lines for QTL Mapping -- Assessing Distribution and Variation of Genome-wide DNA Methylation using Short Read Sequencing -- Circular Chromosome Conformation Capture in Plants -- Genome Wide Profiling of Histone Modifications and Histone Variants in Arabidopsis thaliana and Marchantia polymorpha -- Tissue-Specific Transcriptome Profiling in Arabidopsis Roots -- Sample Preparation Protocols for Protein Abundance, Acetylome and Phosphoproteome Profiling of Plant Tissues -- Automated High-throughput Root Phenotyping of Arabidopsis thaliana under Nutrient Deficiency Conditions -- Large-scale Phenotyping of Root Traits in the Model Legume Lotus japonicas -- Long-term Confocal Imaging of Arabidopsis thaliana Roots for Simultaneous Quantification of Root Growth and Fluorescent Signals -- Identification of Protein-DNA Interactions Using Enhanced Yeast One-Hybrid Assays and a Semi-Automated Approach -- Mapping Protein- protein Interaction using High-throughput Yeast-2-Hybrid -- Mapping Protein-protein Interactions using Affinity Purification and Mass Spectrometry -- Measuring Protein Movement, Oligomerization State, and Protein-Protein Interaction in Arabidopsis Roots Using Scanning Fluorescence Correlation Spectroscopy (scanning FCS) -- Studying Protein-Protein Interactions in planta using Advanced Fluorescence Microscopy -- Chemiluminescence Based Detection of Peptide Activity and Peptide-Receptor Binding in Plants -- Application of Chemical Genomics to Plant-Bacteria Communication: A High-Throughput System to Identify Novel Molecules Modulating the Induction of Bacterial Virulence Genes by Plant Signals.
520 _aThis volume provides protocols that revolve around three pillars of progress in the plant genomics field: genotypes, phenotypes, and the molecular processes in between. Chapters in Plant Genomics: Methods and Protocols are not restricted to the predominant model species Arabidopsis thaliana, hoping to encourage and facilitate other researchers to expand their research to other species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Genomics: Methods and Protocols aims to serve as an inspiration for further studies in plant genomics.
700 1 _aBusch, Wolfgang
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493970018
776 0 8 _iPrinted edition:
_z9781493970025
776 0 8 _iPrinted edition:
_z9781493983643
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7003-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2017
999 _c233896
_d233896