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001			233899
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007			cr nn 008mamaa
008			100301s1995 xxu| s |||| 0|eng d
020			_a9781592595280
024	7		_a10.1385/0896032981 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
100	1		_aKendall, David A. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
245	1	0	_aSignal Transduction Protocols _cby David A. Kendall, Stephen J. Hill.
250			_a1st edition 1995
264		1	_aTotowa, NJ _bHumana Press _c1995
300			_a1 recurso en línea (XI, 307 páginas) _b21 ilustraciones
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v41
505	0		_aThe Problems and Pitfalls of Radioligand Binding -- Solubilization and Purification of a Functional Ionotropic Excitatory Amino Acid Receptor -- Autoradiography of ?1-and ?2-Adrenoceptors -- In Situ Hybridization -- Measurement of the GTPase Activity of Signal-Transducing G-Proteins in Neuronal Membranes -- Measurement of Adenylyl Cyclase Activity in Cell Membranes -- The Measurement of Cyclic AMP Levels in Biological Preparations -- Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation Proximity Assay -- Measurement of Cyclic GMP Formation -- Cyclic AMP-Dependent Protein Kinase Activity Ratio Assay -- Purification and Activation of Cyclic GMP-Dependent Protein Kinase -- The Analysis and Assay of Cyclic Nucleotide Phosphodiesterase Isoenzyme Activity -- Separation of Labeled Inositol Phosphate Isomers by High-Pressure Liquid Chromatography (HPLC) -- Mass Measurement of Key Phosphoinositide Cycle Intermediates -- Measurement of Phospholipase C Activity in Brain Membranes -- Measurement of Diacylglycerol -- Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura-2 -- Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45Ca2+ and Fluo-3 -- Single-Cell Calcium Imaging -- Characterization of Calcium/Calmodulin-Stimulated Protein Kinase II -- Protein Kinase C -- The Measurement of Phospholipase D-Linked Signaling in Cells -- Phospholipase A2 Activity -- Techniques for the Measurement of Nitric Oxide.
520			_aAs our understanding of the biological sciences expands, the bou- aries between traditional disciplines tend to blur at the edges. Physio- gists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and mole- lar biologists. However, the acquisition of new technologies can be a time-consuming and frustrating business, and unless an expert is on hand to give instruction, precious hours can be spent poring over half-described Methods sections with no guarantee of eventual success. The aim of Signal Transduction Protocols has been to get experts with "hands-on" experience in particular techniques to give detailed accounts of experimental protocols in a recipe-type format, which we hope will circumvent the problems of ambiguity often encountered when reading the literature. The techniques described in Signal Transduction Protocols are those that we think will be most useful in addressing questions in the area of receptor-mediated cell signaling, with particular regard to those receptors that are part of the G-protein-linked superfamily. To keep it to a manageable size, we have omitted any reference to electrophysi- ogy and have instead concentrated on more biochemical approaches.
700	1		_aHill, Stephen J. _eautor _4aut _4http://id.loc.gov/vocabulary/relators/aut
776	0	8	_iPrinted edition: _z9781489940681
776	0	8	_iPrinted edition: _z9781489940674
776	0	8	_iPrinted edition: _z9780896032989
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032981 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_1995
999			_c233899 _d233899