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001			233902
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008			161210s2017 xxu| s |||| 0|eng d
020			_a9781493966882
024	7		_a10.1007/978-1-4939-6688-2 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aSynapse Development _bMethods and Protocols _cedited by Alexandros Poulopoulos.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 372 páginas) _b79 ilustraciones, 67 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1538
505	0		_aA Cell Culture System to Investigate the Presynaptic Control of Subsynaptic Membrane Differentiation at the Neuromuscular Junction -- Co-Culture Synaptogenic Assay: A New Look at Fluorescence Reporters and Technological Devices -- Synaptogenic Assays Using Neurons Cultured on Micropatterned Substrates -- Monitoring Synapses Via Trans-Synaptic GFP Complementation -- Generation of Spinal Motor Neurons from Human Pluripotent Stem Cells -- Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins -- In Situ Protein Binding Assay Using Fc-Fusion Proteins -- ation Microscopy for the Investigation of Synaptic Structure and Function -- 3D dSTORM Imaging of Fixed Brain Tissue -- Photomarking Relocalization Technique for Correlated Two-Photon and Electron Microcopy Imaging of Single Stimulated Synapses -- 3D Analysis of Synaptic Ultrastructure in Organotypic Hippocampal Slice Culture by High-Pressure Freezing and Electron Tomography -- Analyzing Endosomal Docking, Fusion, Sorting, and Budding Mechanisms in Isolated Organelles -- Concurrent Imaging of Receptor Trafficking and Calcium Dynamics by Spinning Disk Confocal Microscopy -- Imaging Activity-Dependent Signaling Dynamics at the Neuronal Synapse Using FRET-Based Biosensors -- Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture -- Using Fluorescent Markers to Estimate Synaptic Connectivity In Situ -- Dual Anterograde and Retrograde Viral Tracing of Reciprocal Connectivity -- Mapping Synaptic Inputs of Developing Neurons Using Calcium Imaging -- Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus.
520			_aThis detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the brain and in culture, their constituents, their structures, their dynamics, and the assemblies they form, all in the structure of a laboratory guide. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synapse Development: Methods and Protocols serves as an ideal guide to minimizing the barrier to entry for the integration of new approaches with existing expertise, producing syntheses that will foster novel perspectives on the many ways in which synapses form, transform, and transmit.
700	1		_aPoulopoulos, Alexandros _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493966868
776	0	8	_iPrinted edition: _z9781493966875
776	0	8	_iPrinted edition: _z9781493982738
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6688-2 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233902 _d233902