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008			100301s2002 xxu| s |||| 0|eng d
020			_a9781592593408
024	7		_a10.1385/1592593402 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTransgenic Mouse Methods and Protocols _cedited by Marten H. Hofker, Jan van Deursen.
250			_a1st edition 2002
264		1	_aTotowa, NJ _bHumana Press _c2002
300			_a1 recurso en línea (392 páginas) _b80 ilustraciones, 4 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v209
505	0		_aIntroduction: The Use of Transgenic Mice in Biomedical Research -- Genetic Modification of the Mouse -- In Vitro Fertilization/Cryopreservation -- Transgene Design -- Generating Conditional Mouse Mutants via Tetracycline-Controlled Gene Expression -- Manipulating Large Insert Clones for Transgenesis -- Reporter Molecules in Genetically Engineered Mice -- Gene Targeting in Mouse Embryonic Stem Cells -- Conditional Knockout Mice -- Knockin Approaches -- Aggregation of Embryos and Embryonic Stem Cells -- Andenovirus-Mediated Gene Transfer -- Random ENU Mutagenesis -- Autopsy and Histologic Analysis of the Transgenic Mouse -- Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells -- Quantification of Atherosclerosis in Mice -- Genetically Modified Mice in Cancer Research -- Neuropathobiology in Transgenic Mice.
520			_aGenetically very similar to the human species, mice play an important role in biomedical research and have served as experimental models for a wide variety of pathologies, including cancer, cardiovascular diseases, and behavioral disorders. In Transgenic Mouse Methods and Protocols, Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularly for generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented. Care is taken to make clear the details of the available approaches, as well as their limitations. Up-to-date and highly practical, Transgenic Mouse Methods and Protocols demonstrates clearly for both novice and expert investigators how to make novel transgenic mouse models, and how to use them effectively to understand the role of gene function in human health and disease.
700	1		_aHofker, Marten H _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_avan Deursen, Jan _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781489938312
776	0	8	_iPrinted edition: _z9780896039155
776	0	8	_iPrinted edition: _z9781489938305
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592593402 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2002
999			_c233912 _d233912