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020 _a9781592597611
024 7 _a10.1385/1592597610
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aGenetic Recombination
_bReviews and Protocols
_cedited by Alan S. Waldman.
250 _a1st edition 2004
264 1 _aTotowa, NJ
_bHumana Press
_c2004
300 _a1 recurso en línea (X, 260 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v262
505 0 _aStudying Recombination Events in Eukaryotes -- Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae -- Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells -- Intrachromosomal Homologous Recombination in Arabidopsis thaliana -- Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells With I-SceI Nuclease -- Transformation of Monomorphic and Pleomorphic Trypanosoma brucei -- Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice -- Recombination as a Reporter of Genomic Instability -- Detecting Carcinogens With the Yeast DEL Assay -- In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer -- Recombination as a Tool for Producing Targeted Genetic Modification -- Gene Targeting at the Chromosomal Immunoglobulin Locus -- DNA Fragment Transplacement in Saccharomyces cerevisiae -- Targeted Gene Modification Using Triplex-Forming Oligonucleotides -- Using Nucleases to Stimulate Homologous Recombination -- Enhancement of In Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA -- Biochemistry of Recombination -- Chromatin Immunoprecipitation to Investigate Protein-DNA Interactions During Genetic Recombination -- Holliday Junction Branch Migration and Resolution Assays.
520 _aGenetic recombination plays an essential role in such vitally important biological processes as DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. In Genetic Recombination: Reviews and Protocols, prominent international investigators actively engaged in recombination research describe in great detail their best techniques for studying recombination. The methods range from approaches and model systems to be used in a variety of eukaryotic organisms and in a mammalian parasite, to biochemical analyses useful for furthering the understanding of recombination mechanisms. Additional techniques use recombination as a reporter of genomic instability in lower and higher eukaryotes and as a tool for producing targeted genetic modification. Each readily reproducible method includes step-by-step instructions, a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and highly practical, Genetic Recombination: Reviews and Protocols offers both novice and experienced investigators an illuminating synthesis of what we currently know about recombination, as well as a wide-ranging collection of powerful tools for elucidating its mechanisms and regulation throughout biology today.
700 1 _aWaldman, Alan S
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617374425
776 0 8 _iPrinted edition:
_z9781588292360
776 0 8 _iPrinted edition:
_z9781489939296
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597610
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2004
999 _c233922
_d233922