000			04694nam a22003615i 4500
001			233924
003			ES-VaUE
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007			cr nn 008mamaa
008			180212s2018 xxu| s |||| 0|eng d
020			_a9781493976010
024	7		_a10.1007/978-1-4939-7601-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aMicroRNA Protocols _cedited by Shao-Yao Ying.
250			_a3rd edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XI, 319 páginas) _b50 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1733
505	0		_aThe MicroRNA.- Target mRNA Driven Biogenesis of Cognate MicroRNAs In Vitro.- Isolation of Viral-Infected Brain Regions for miRNA Profiling from Formalin-Fixed Paraffin-Embedded Tissues by Laser Capture Microdissection .- Isolation and Analysis of Exosomal Microsomal MicroRNAs from Ovarian Follicular Fluid.- Profiling of MicroRNAs in the Biofluids of Livestock Species.- Exosomal MicroRNAs as Potential Biomarkers in Neuropsychiatric Disorders -- Identification and Validation of Potential Differential miRNA Regulation via Alternative Polyadenylation.- How to Explore the Function and Importance of MicroRNAs: MicroRNAs Expression Profile and their Target/Pathway Prediction in Bovine Ovarian Cells.- Gene Silencing In Vitro and In Vivo using Intronic MicroRNAs.- Mining Exosomal MicroRNAs from Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes for Cardiac Regeneration.- Quantitative Analysis of Precursors MicroRNAs and their Respective Mature MicroRNAs in Cancer Exosomes Overtime.- Quantum Language of MicroRNA: Application for New Cancer Therapeutic Targets -- In Vitro Methods for Analyzing miRNA Roles in Cancer Cell Proliferation, Invasion,  and Metastasis.- Isolation and Identification of Gene-Specific MicroRNAs.- Comprehensive Measurement of Gene Silencing Involving Endogenous MicroRNAs in Mammalian Cells -- Screening miRNA for Functional Significance by 3D Cell Culture System.- Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs.- Neonatal Rat Cardiomyocytes Isolation, Culture and Determination of MicroRNAs' Effects in Proliferation.- Gene Manipulation with Micro RNAs at Single Human Cancer Cell.- Laser Capture Microdissection of Epithelium from a Wound Healing Model for MicroRNA Analysis.- Transgene-Like Animal Models Using Intronic MicroRNAs -- Application of TALE-Based Approach for Dissecting Functional MicroRNA-302/367 in Cellular Reprogramming.- Mechanism and Method for Generating Tumor-Free iPS Cells Using Intronic MicroRNA miR302 Induction.- The miR-302-Mediated Induction of Pluripotent Stem Cells (iPSC): Multiple Synergistic Reprogramming Mechanisms.- Identification and Isolation of Novel Sugar-Like RNA Protecting Materials Glycylglycerins from Pluripotent Stem Cells.
520			_aThis volume focuses on the analysis of miRNA and its many components such as targets and expression profiling, regulation of gene expression, miRNA detection in biofluids, and its application in cancer and wound healing. The chapters in this book explore topics that's cover biogenesis, isolation and profiling of miRNA, exosomal miRNAs, and the study of the functional significance of miRNAs. The chapters also cover screening miRNAs in mR-302/367 induced iPSCs, exosomal miRNAs from iPSC-derived cardiomyocytes proliferation, and the recently discovered sugar-like RNA glycylglycerins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, MicroRNA Protocols, Third Edition is a valuable resource that provides researchers with a comprehensive study of miRNAs and its numerous components and uses.
700	1		_aYing, Shao-Yao _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493976003
776	0	8	_iPrinted edition: _z9781493976027
776	0	8	_iPrinted edition: _z9781493992508
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7601-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233924 _d233924