000			04541nam a22003735i 4500
001			233926
003			ES-VaUE
005			20221220020531.0
007			cr nn 008mamaa
008			180310s2018 xxu| s |||| 0|eng d
020			_a9781493977475
024	7		_a10.1007/978-1-4939-7747-5 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRoot Development _bMethods and Protocols _cedited by Daniela Ristova, Elke Barbez.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 324 páginas) _b83 ilustraciones, 65 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1761
505	0		_aMeasuring Plant Root Traits Under Controlled and Field Conditions: Step-By-Step Procedures -- Phenotyping Crop Root Crowns: General Guidance and Specific Protocols for Maize, Wheat, and Soybean -- Developmental Analysis of Arabidopsis Root Meristem -- Genetic and Phenotypic Analysis of Lateral Root Development in Arabidopsis thaliana -- Adapting the Lateral Root Inducible System to Medicago truncatula -- Characterization of Root Epidermal Cell Patterning and differentiation in Arabidopsis -- In Vitro Assay for Induction of Adventitious Rooting on Intact Arabidopsis Hypocotyls -- Root Gravitropism: Quantification, Challenges, and Solutions -- Calcium Ion Dynamics in Roots: Imaging and Analysis -- Optimized Whole-Mount In Situ Immunolocalization for Arabidopsis thaliana Root Meristems and Lateral Root Primordia -- Light Sheet Fluorescence Microscopy Optimized for Long-Term Imaging of Arabidopsis Root Development -- Histological Profiling Over Time to Optimize Root Cell Type-Specific Reporter Lines for Cell Sorting -- Long-Term In Vivo Imaging of Luciferase-Based Reporter Gene Expression in Arabidopsis Roots -- Cortical Cell Length Analysis During Gravitropic Root Growth -- Growth Rate Normalization Method to Assess Gravitropic Root Growth -- Immunoprecipitation of Membrane Proteins from Arabidopsis thaliana Root Tissue -- Monitoring Transcriptomic Changes in Soil-Grown Roots and Shoots of Arabidopsis thaliana Subjected to a Progressive Drought Stress -- Chromatin Immunoprecipitation Sequencing (ChIP-Seq) for Transcription Factors and Chromatin Factors in Arabidopsis thaliana Roots: From Material Collection to Data Analysis -- μChIP-Seq for Genome-Wide Mapping of In Vivo TF-DNA Interactions in Arabidopsis Root Protoplasts -- Proteome Analysis of Arabidopsis Roots -- Step-By-Step Construction of Gene Co-Expression Networks from High-Throughput Arabidopsis RNA Sequencing Data -- GWA-Portal: Genome-Wide Association Studies Made Easy.
520			_aThis detailed volume provides diverse elegant methods, complemented with existing protocols, which are optimized for the current needs in plant root biology as well as for use in plant species other than Arabidopsis thaliana. The collection covers methods ranging from genetic screens, phenotypic analysis, and cell biology methods to systems biology tools and genome-wide approaches. The collection contains a range of complexity from fundamental methods for quantification of different root developmental processes to complex methods that require sophisticated equipment. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Root Development: Methods and Protocols serves as a valuable tool for plant biologists specialized in root development, from beginners to experienced researchers in the field.
700	1		_aRistova, Daniela _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aBarbez, Elke _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493977468
776	0	8	_iPrinted edition: _z9781493977482
776	0	8	_iPrinted edition: _z9781493992737
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7747-5 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233926 _d233926