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020 _a9781493978342
024 7 _a10.1007/978-1-4939-7834-2
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aGene Expression Analysis
_bMethods and Protocols
_cedited by Nalini Raghavachari, Natàlia Garcia-Reyero.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XI, 387 páginas)
_b84 ilustraciones, 72 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1783
505 0 _aOverview of Gene Expression Analysis - Transcriptomics -- RNA-Seq and Expression Arrays: Selection Guidelines for Genome-wide Expression Profiling -- A Guide for Designing and Analysing RNA-Seq Data -- SureSelectXT RNA Direct: A Technique for Expression Analysis Through Sequencing of Target-enriched FFPE total RNA -- Simultaneous, Multiplexed Detection of RNA and Protein on the NanoString® nCounter® Platform -- Transcript Profiling Using Long-read Sequencing Technologies -- Making and Sequencing Heavily-Multiplexed, High-throughput 16S Ribosomal RNA Gene Amplicon Libraries Using a Flexible, Two-stage PCR PROTOCOL -- microRNA Expression Analysis - Next Generation Sequencing -- Identification of Transcriptional Regulators that Bind to Long Noncoding RNAs by RNA Pull Down and RNA Immunoprecipitation -- Single-cell mRNA-Seq using the Fluidigm C1 System and Integrated Fluidics Circuits -- Current and Future Methods for mRNA Analysis: A Drive Towards Single Molecule Sequencing -- Expression Profiling of Differentially Regulated Genes in Fanconi Anemia -- A Review of Transcriptome Analysis in Pulmonary Vascular Diseases -- Differential Gene Expression Analysis of Plants -- High Throughput Sequencing Based Approaches for Gene Expression Analysis -- Network Analysis of Gene Expression -- Analysis of ChIP-Seq and RNA-Seq data with BioWardrobe -- Bayesian Network to Infer Drug-induced Apoptosis Circuits from Connectivity Map Data. .
520 _aThis volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics techniques, and targeted sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Gene Expression Analysis: Methods and Protocols aims provide useful information to researchers worldwide.
700 1 _aRaghavachari, Nalini
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aGarcia-Reyero, Natàlia
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493978335
776 0 8 _iPrinted edition:
_z9781493978359
776 0 8 _iPrinted edition:
_z9781493992997
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7834-2
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c233935
_d233935