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020 _a9781493978021
024 7 _a10.1007/978-1-4939-7802-1
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPlanarian Regeneration
_bMethods and Protocols
_cedited by Jochen C. Rink.
250 _a1st edition 2018
264 1 _aNew York, NY
_bSpringer International Publishing
_c2018
300 _a1 recurso en línea (XI, 573 páginas)
_b92 ilustraciones, 56 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1774
505 0 _aPlanarian Diversity and Phylogeny -- Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale -- The Ecology of Freshwater Planarians -- Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology -- Culturing Planarians in the Laboratory -- Total RNA Isolation from Planarian Tissues -- Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians -- Planarian High Molecular Weight DNA Isolation by Spooling -- Basic Histological Techniques for Planarians -- Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians -- Immunohistochemistry on Paraffin-Embedded Planarian Tissue Sections -- Whole-Mount In Situ Hybridization of Planarians -- RNA In Situ Hybridization on Planarian Paraffin Sections -- General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohistochemistry Methods -- Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians -- Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL -- Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria -- RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA -- A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells -- Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and Single-Cell Transplantation -- Tissue Transplantations in Planarians -- Live Imaging Planaria -- Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques -- Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC -- Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.
520 _aThis volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental protocols for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Comprehensive and cutting-edge, Planarian Regeneration: Methods and Protocols is a valuable resource for both newcomers to the field and experts within established planarian laboratories.
700 1 _aRink, Jochen C
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493978007
776 0 8 _iPrinted edition:
_z9781493978014
776 0 8 _iPrinted edition:
_z9781493992898
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7802-1
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2018
999 _c233940
_d233940