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001			233941
003			ES-VaUE
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007			cr nn 008mamaa
008			180618s2018 xxu| s |||| 0|eng d
020			_a9781493978564
024	7		_a10.1007/978-1-4939-7856-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Vacuolar Trafficking _bMethods and Protocols _cedited by Cláudia Pereira.
250			_a1st edition 2018
264		1	_aNew York, NY _bSpringer International Publishing _c2018
300			_a1 recurso en línea (XII, 222 páginas) _b51 ilustraciones, 41 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1789
505	0		_aGreen-fluorescent Seed, to Evaluate Vacuolar Trafficking in Arabidopsis Seeds -- Detection of CRISPR/Cas9 Induced Genomic Fragment Deletions in Barley and Generation of Homozygous Edited Lines via Embryogenic Pollen Culture -- Vacuolar Sorting Determinants - Isolation and Study -- Vacuole-targeted Proteins: Ins and Outs of Subcellular Localization Studies -- Plant Cell Vacuoles: Staining and Fluorescent Probes -- Vacuolar Targeting and Characterization of Recombinant Antibodies -- Isolation of Vacuoles from the Leaves of the Medicinal Plant Catharanthus roseus -- Flow Cytometry and Fluorescence Microscopy as Tools for Structural and Functional Analysis of Vacuoles Isolated from Yeast and Plant Cells -- Live cell Imaging and Confocal Microscopy -- Imaging Vacuolar Anthocyanins with Fluorescence Lifetime Microscopy (FLIM) -- The Use of Drugs in the Study of Vacuole Morphology and Trafficking to the Vacuole in Arabidopsis thaliana -- Use of Brefeldin A and Wortmannin to Dissect Post-Golgi Organelles Related to Vacuolar Transport in Arabidopsis thaliana -- Use Endosidin2 to Study Plant Exocytosis and Vacuolar Trafficking -- Visualization of RMRs (Receptor Membrane RING-H2) Dimerization in Nicotiana Benthamiana Leaves using a Bimolecular Fluorescence Complementation (BiFC) Assay -- Vacuole Dynamics in Rice Cells Invaded by the Blast Fungus Magnaporthe Oryzae -- Characterization Of Plant Glycoproteins: Analysis of Plant Glycopeptide Mass Spectrometry Data with plantGlycoMS, a Package in the R Statistical Computing Environment.
520			_aThis volume provides a collection of state-of-the-art protocols on plant vacuolar trafficking. Chapters guide the reader through design and preparation of fusion proteins, expression vectors, and detailed analysis of different microscopy techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Vacuolar Trafficking: Methods and Protocols aims to be a highly valuable resource for researchers interested in learning more about this field.
700	1		_aPereira, Cláudia _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493978557
776	0	8	_iPrinted edition: _z9781493978571
776	0	8	_iPrinted edition: _z9781493993055
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-7856-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2018
999			_c233941 _d233941