000			03509nam a22003495i 4500
001			233949
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007			cr nn 008mamaa
008			100301s2008 xxu| s |||| 0|eng d
020			_a9781597451918
024	7		_a10.1007/978-1-59745-191-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRNAi _bDesign and Application _cedited by Sailen Barik.
250			_a1st edition 2008
264		1	_aTotowa, NJ _bHumana Press _c2008
300			_a1 recurso en línea (XIV, 270 páginas) _b48 ilustraciones, 3 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v442
505	0		_aApplication of RNAi in Diverse Organisms -- Principles of Dicer Substrate (D-siRNA) Design and Function -- Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes -- In vitro RNA Cleavage Assay for Argonaute-Family Proteins -- Identifying siRNA-Induced Off-Targets by Microarray Analysis -- Application of RNAi in Diverse Organisms -- Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis -- Nasal Delivery of siRNA -- RNA Interference as a Genetic Tool in Trypanosomes -- Lentivirus-Mediated RNA Interference in Mammalian Neurons -- Silencing Genes by RNA Interference in the Protozoan Parasite Entamoeba histolytica -- Use of RNAi in C. elegans -- Application of siRNA Against SARS in the Rhesus Macaque Model -- siRNA and shRNA as Anticancer Agents in a Cervical Cancer Model -- Identification and Expression Analysis of Small RNAs During Development -- Screening and Identification of Virus-Encoded RNA Silencing Suppressors -- Application of RNA Interference in Functional Genomics Studies of a Social Insect -- Temporal Control of Gene Silencing by in ovo Electroporation -- Altering Flower Color in Transgenic Plants by RNAi-Mediated Engineering of Flavonoid Biosynthetic Pathway -- Transgenic RNA Interference in Mice.
520			_aRNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.
700	1		_aBarik, Sailen _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617378195
776	0	8	_iPrinted edition: _z9781588298744
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-59745-191-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2008
999			_c233949 _d233949