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008			161024s2017 xxu| s |||| 0|eng d
020			_a9781493964390
024	7		_a10.1007/978-1-4939-6439-0 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aActivity-Based Proteomics _bMethods and Protocols _cedited by Herman S. Overkleeft, Bogdan I. Florea.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 222 páginas) _b47 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1491
505	0		_aActivity-Based Protein Profiling - From Chemical Novelty to Biomedical Stalwart -- A Quantitative Mass-Spectrometry Platform to Monitor Changes in Cysteine Reactivity -- Activity-Based Protein Profiling with Natural Product-Derived Chemical Probes in Human Cell Lysates -- Inhibitor Discovery by Convolution Activity-Based Protein Profiling -- Activity-Based Protein Profiling in Bacteria -- Activity-Based Lipid Esterase Profiling of M. Bovis BCG at Different Metabolic States Using Tetrahydrolipstatin (THL) as Bait -- Comparative Activity-Based Flavin-Dependent Oxidase Profiling -- Activity-Dependent Photoaffinity Labelling of Metalloproteases -- Profiling the Activity of Deubiquitinating Enzymes using Chemically Synthesized Ubiquitin-Based Probes -- Profiling Protein Sumoylation using Biochemical and Mass Spectrometry-Based Research Tools -- Live Cell Imaging and Profiling of Cysteine Cathepsin Activity using a Quenched Activity-Based Probe -- Competitive Activity-Based Protein Profiling Of Serine Hydrolases: A Case Study on DAGL-Alpha -- A Protocol for Protein Profiling using Chemoselective Cleavable Linker Probes in Semi-Permeabilized Cells -- Cleavable Linkers in Chemical Proteomics Applications -- Two-Step Activity-Based Protein Profiling With the Proteasome System as Model of Study.
520			_aThis volume focuses on explorative activity-based proteomics, biomedical applications of activity-based proteomics, and chemical strategies in activity-based proteomics providing a concise overview of activity-based protein profiling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Activity-Based Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.
700	1		_aOverkleeft, Herman S _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aFlorea, Bogdan I _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493964376
776	0	8	_iPrinted edition: _z9781493964383
776	0	8	_iPrinted edition: _z9781493982004
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6439-0 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233955 _d233955