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020 _a9781493930340
024 7 _a10.1007/978-1-4939-3034-0
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aPatient-Specific Induced Pluripotent Stem Cell Models
_bGeneration and Characterization
_cedited by Andras Nagy, Kursad Turksen.
250 _a1st edition 2016
264 1 _aNew York, NY
_bSpringer International Publishing
_c2016
300 _a1 recurso en línea (XIII, 368 páginas)
_b72 ilustraciones, 52 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v1353
505 0 _aGeneration of Patient-Specific Induced Pluripotent Stem Cell from Peripheral Blood Mononuclear Cells by Sendai Reprogramming Vectors -- A Doxycycline-Inducible System for Genetic Correction of iPSC Disease Models -- Generation and Characterization Of Patient-Specific Induced Pluripotent Stem Cell For Disease Modeling -- Modeling Genomic Imprinting Disorders Using Induced Pluripotent Stem Cells -- Generation and Characterization of Induced Pluripotent Stem Cells from Patients with mtDNA Mutations -- Skin Biopsy and Patient-Specific Stem Cell Lines -- Directed Myogenic Differentiation of Human Induced Pluripotent Stem Cells -- Using Human Induced Pluripotent Stem Cells to Model Skeletal Diseases -- Modeling Cardiovascular Diseases with Patient-Specific Human Pluripotent Stem Cell-Derived Cardiomyocytes -- Calcium Imaging in Pluripotent Stem Cell-Derived Cardiac Myocytes -- Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization of Cardiac Cells -- Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes Under Defined Conditions -- Generation of Cardiomyocytes from Pluripotent Stem Cells -- Generation and Characterization of Patient-Specific iPSC-Model for Cardiovascular Disease -- Transgene-Free Disease-Specific iPSC Generation from Fibroblasts and Peripheral Blood Mononuclear Cells -- Generation and Neuronal Differentiation of Patient-Specific Induced Pluripotent Stem Cells Derived from Niemann-Pick Type C1 Fibroblasts -- Multisystemic Disease Modeling of Liver-Derived Protein Folding Disorders Using Induced Pluripotent Stem Cells (iPSCs) -- In Vitro Modeling of Alcohol-Induced Liver Injury Using Human Induced Pluripotent Stem Cells -- Generation of Human Induced Pluripotent Stem Cells Using RNA-Based Sendai Virus System and Pluripotency Validation of the Resulting Cell Population -- Modeling Axonal Phenotypes with Human Pluripotent Stem Cells -- Mitochondrial Disease-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization -- Patient-Specific Induced Pluripotent Stem Cell Models: Characterization of iPS Cell-Derived Cardiomyocytes -- Generation of Integration-Free Patient Specific iPS Cells Using Episomal Plasmids Under Feeder Free Conditions.
520 _aThis volume captures the rapid developments in the field of induced pluripotent stem (iPS) cells, which have provided novel opportunities and approaches both for better understanding a number of human diseases and for developing new platforms for drug development and screening for such diseases. Specifically, representative protocols on various disease models have been collected from labs around the world. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Patient-Specific Induced Pluripotent Stem Cell Models: Generation and Characterization is an ideal reference for scientists working on furthering iPS research.
700 1 _aNagy, Andras
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
700 1 _aTurksen, Kursad
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781493930333
776 0 8 _iPrinted edition:
_z9781493930357
776 0 8 _iPrinted edition:
_z9781493949731
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-3034-0
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2016
999 _c233962
_d233962