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008 100327s2010 xxu| s |||| 0|eng d
020 _a9781607616917
024 7 _a10.1007/978-1-60761-691-7
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aCellular Programming and Reprogramming
_bMethods and Protocols
_cedited by Sheng Ding.
250 _a1st edition 2010
264 1 _aTotowa, NJ
_bHumana Press
_c2010
300 _a1 recurso en línea (XII, 334 páginas)
_b54 ilustraciones, 4 ilustraciones a color
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v636
505 0 _aHuman Embryonic Stem Cell Derivation, Maintenance, and Differentiation to Trophoblast -- Isolation and Maintenance of Mouse Epiblast Stem Cells -- Functional Assays for Hematopoietic Stem Cell Self-Renewal -- Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells from Rat Bone Marrow -- Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In Vitro -- Mesoderm Cell Development from ES Cells -- Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells -- Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs -- Directing Human Embryonic Stem Cells to a Retinal Fate -- Bovine Somatic Cell Nuclear Transfer -- Cell Fusion-Induced Reprogramming -- An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells -- Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic Cells -- Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor Expression -- Reprogramming of B Cells -- Adult Cell Fate Reprogramming: Converting Liver to Pancreas -- In Vitro Reprogramming of Pancreatic Cells to Hepatocytes -- Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches -- Small Molecule Screen in Zebrafish and HSC Expansion -- Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation.
520 _aBefore the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body's own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls. Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer.
700 1 _aDing, Sheng
_eeditor literario
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781607616924
776 0 8 _iPrinted edition:
_z9781607616900
776 0 8 _iPrinted edition:
_z9781493957149
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-60761-691-7
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2010
999 _c233972
_d233972