000			03237nam a22003615i 4500
001			233973
003			ES-VaUE
005			20221220020533.0
007			cr nn 008mamaa
008			110506s2011 xxu| s |||| 0|eng d
020			_a9781617791147
024	7		_a10.1007/978-1-61779-114-7 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aRNA Interference Techniques _cedited by Scott Q. Harper.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XII, 174 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aNeuromethods _x1940-6045 _v58
505	0		_aDesigning Hairpin-Based RNAi Shuttles -- Rapid Cloning and Validation of MicroRNA Shuttle Vectors:  A Practical Guide -- The Use of Small Non-Coding RNAs to Silence Transcription in Human Cells -- Delivery Strategies for RNAi to the Nervous System -- Cloning Small RNAs -- Profiling the miRNome: Detecting Global miRNA Expression Levels with DNA Microarrays -- High Throughput Profiling of Mature MicroRNA by Real-Time PCR -- In Situ Detection of MicroRNAs in Paraffin Embedded, Formalin Fixed Tissues: Different Methodologies and Co-Localization with Possible Targets -- Tracing of siRNAs Inside Cells by FRET Imaging -- Application of RIP-Chip for the Identification of miRNA Targets.
520			_aNon-coding, inhibitory microRNAs have emerged as important modulators of cellular gene expression, through a process called RNA interference (RNAi).  To date, hundreds of conserved and species-specific microRNAs have been identified in organisms ranging from single-celled algae to humans.  Many of these tiny RNAs are now known to play fundamental roles in developmental biology and disease pathogenesis.  In addition, RNAi has emerged as a technology useful for manipulating gene expression.  In RNA Interference Techniques, expert researchers present detailed methods for designing and delivering artificial inhibitory RNAs to neural tissue and for detecting or cloning endogenous microRNAs, all in order to aid investigators' attempts to ask basic biological questions or develop therapeutics for dominant neurogenetic disorders, cancer, or viral infection. As a volume in the successful Neuromethods series, the chapters provide authoritative accounts of the most commonly used approaches in the field today.   Cutting-edge and concise, RNA Interference Techniques promises to support the vital research in the field of RNAi and miRNAs, ever-continuing to grow rapidly and gain increasing importance in basic and translational biology.
700	1		_aHarper, Scott Q _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617791130
776	0	8	_iPrinted edition: _z9781617791154
776	0	8	_iPrinted edition: _z9781493957620
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61779-114-7 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c233973 _d233973