| 000 | 03094nam a22003615i 4500 | ||
|---|---|---|---|
| 001 | 233974 | ||
| 003 | ES-VaUE | ||
| 005 | 20221220020533.0 | ||
| 007 | cr nn 008mamaa | ||
| 008 | 100301s2004 xxu| s |||| 0|eng d | ||
| 020 | _a9781592597512 | ||
| 024 | 7 |
_a10.1385/1592597513 _2doi |
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| 040 |
_aES-MaUEC _bspa _cES-MaUEC |
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| 245 | 1 | 0 |
_aGene Expression Profiling _cedited by Richard A. Shimkets. |
| 250 | _a1st edition 2004 | ||
| 264 | 1 |
_aTotowa, NJ _bHumana Press _c2004 |
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| 300 |
_a1 recurso en línea (X, 168 páginas) _b |
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| 336 |
_atexto _btxt _2rdacontent |
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| 337 |
_aelectrónico _bc _2rdamedia |
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| 338 |
_arecurso electrónico _bcr _2rdacarrier |
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| 347 |
_aarchivo de texto _bPDF |
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| 490 | 0 |
_aMethods in Molecular Biology _x1940-6029 _v258 |
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| 505 | 0 | _aTechnical Considerations in Quantitating Gene Expression -- Gene Expression Quantitation Technology Summary -- Standardized RT-PCR and the Standardized Expression Measurement Center -- GeneCalling -- Invader Assay for RNA Quantitation -- Monitoring Eukaryotic Gene Expression Using Oligonucleotide Microarrays -- Amplified Differential Gene Expression Microarray -- Suppression Subtractive Hybridization -- Small Amplified RNA-SAGE -- Gene Expression Informatics. | |
| 520 | _aThe transcription of messenger RNA from a DNA template is a key process in a wide variety of biological systems. In Gene Expression Profiling: Methods and Protocols, leaders in gene expression methodology and bioinformatics data analysis share their best methods for measuring RNA levels in cells and tissues. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Special care has been taken to distill the basic underlying principles of each method to a few straightforward concepts so that investigators can easily choose the method most appropriate to their application. The readily reproducible techniques presented include new methods for applying the GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. Cutting-edge and highly practical, Gene Expression Profiling: Methods and Protocols allows novice and experienced investigators alike not only to choose the best method to determine how genes are turned on and off in many different organisms, but also to use informatics resources to analyze and understand the results. | ||
| 700 | 1 |
_aShimkets, Richard A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt |
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| 776 | 0 | 8 |
_iPrinted edition: _z9781617374296 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781489939616 |
| 776 | 0 | 8 |
_iPrinted edition: _z9781588292209 |
| 856 | 4 | 0 |
_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592597513 _z(usuarios Universidad Europea de Valencia) |
| 942 |
_2lcc _cLE |
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| 988 | _aSpringer_Protocols_2004 | ||
| 999 |
_c233974 _d233974 |
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