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008			161122s2017 xxu| s |||| 0|eng d
020			_a9781493966349
024	7		_a10.1007/978-1-4939-6634-9 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aAntibiotics _bMethods and Protocols _cedited by Peter Sass.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 358 páginas) _b78 ilustraciones, 21 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1520
505	0		_aAntibiotics - Precious Goods in Changing Times -- Mining Bacterial Genomes for Secondary Metabolite Gene Clusters -- Production of Antimicrobial Compounds by Fermentation -- Structure Elucidation of Antibiotics by NMR Spectroscopy -- Computer-Aided Drug Design Methods -- Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents -- Application of a Bacillus subtilis Whole-cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds -- Determination of Bacterial Membrane Impairment by Antimicrobial Agents -- Mass-sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes -- Measurement of Cell Membrane Fluidity by Laurdan GP:Fluorescence Spectroscopy and Microscopy -- In vitro Assays to Identify Antibiotics Targeting DNA Metabolism -- Fluorescence-based real-time Activity Assays to Identify RNase P inhibitors -- Reporter Gene-based Screening for TPP Riboswitch Activators -- Cell-based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation.-Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen -- Expression Profiling of Antibiotic Resistant Bacteria Obtained by Laboratory Evolution -- Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research -- Label-Free Quantitation of Ribosomal Proteins from Bacillus Subtillis for Antibiotic Research -- Functional Metagenomics to Study Antibiotic Resistance -- Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains using High Throughput Sequencing. .
520			_aThis volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods and Protocols guides readers through chapters on production and design, mode of action, and response and susceptibility. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Antibiotics: Methods and Protocols aims to inspire scientific work in the exciting field of antibiotic research. .
700	1		_aSass, Peter _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493966325
776	0	8	_iPrinted edition: _z9781493966332
776	0	8	_iPrinted edition: _z9781493982554
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6634-9 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233976 _d233976