000			03901nam a22003615i 4500
001			233989
003			ES-VaUE
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007			cr nn 008mamaa
008			101222s2011 xxu| s |||| 0|eng d
020			_a9781617379574
024	7		_a10.1007/978-1-61737-957-4 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aPlant Chromosome Engineering _bMethods and Protocols _cedited by James A. Birchler.
250			_a1st edition 2011
264		1	_aTotowa, NJ _bHumana Press _c2011
300			_a1 recurso en línea (XI, 340 páginas) _b
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v701
505	0		_aRecent Advances in Plant Transformation -- Engineering the Plastid Genome of Nicotiana sylvestris, a Diploid Model Species for Plastid Genetics -- Homologous Recombination in Plants: An Anti-Review -- Chromosome Painting for Plant Biotechnology -- Plant B Chromosomes -- Telomere Truncation in Plants -- Engineered Plant Minichromosomes -- Method for Bxb1-Mediated Site-Specific Integration In planta -- Targeted Mutagenesis in Arabidopsis Using Zinc Finger Nucleases -- Vectors and Methods for Hairpin RNA and Artificial microRNA-Mediated Gene Silencing in Plants -- Recombinant Protein Expression in Nicotiana -- Chromosome Analysis and Sorting Using Flow Cytometry -- Super-Stretched Pachytene Chromosomes for Plant Molecular Cytogenetic Mapping -- Cytological Dissection of the Triticeae Chromosomes by the Gametocidal System -- Development and Use of Oat-Maize Chromosome Additions and Radiation Hybrids -- Enhancer Trapping in Plants -- Chromatin Beacons: Global Sampling of Chromatin Physical Properties Using Chromatin Charting Lines -- Transposable Elements as Catalysts for Chromosome Rearrangements.
520			_aWith an increasing human population and a decreasing amount of arable land, creative improvements in agriculture will be a necessity in the coming decades to maintain or improve the standard of living. In Plant Chromosome Engineering: Methods and Protocols, expert researchers present techniques for the modification of crops and other plant species in order to achieve the goal of developing the much needed novel approaches to the production of food, feed, fuel, fiber, and pharmaceuticals. This volume examines vital topics such as transformation procedures, chromosome painting, production of engineered minichromosomes, gene targeting and mutagenesis, site specific integration, gene silencing, protein expression, chromosome sorting and analysis, protocols for generating chromosomal rearrangements, enhancer trapping, and means of studying chromosomes in vivo. As a part of the highly successful Methods in Molecular Biology™ series, the methodological chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and professional tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Plant Chromosome Engineering: Methods and Protocols highlights the spectrum of tools currently available for modifying plant genomes and chromosomes and provides the foundation for crucial future developments.
700	1		_aBirchler, James A _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781617379567
776	0	8	_iPrinted edition: _z9781617379581
776	0	8	_iPrinted edition: _z9781493957972
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-61737-957-4 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2011
999			_c233989 _d233989