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020 _a9781592598588
024 7 _a10.1385/1592598587
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
245 1 0 _aProtein Nanotechnology
_bProtocols, Instrumentation, and Applications
_cedited by Tuan Vo-Dinh.
250 _a1st edition 2005
264 1 _aTotowa, NJ
_bHumana Press
_c2005
300 _a1 recurso en línea (XIV, 466 páginas)
_b195 ilustraciones
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v300
505 0 _aProtein Nanotechnology -- Kinetics and Mechanisms of Protein Crystallization at the Molecular Level -- Nanostructured Systems for Biological Materials -- Nanomaterials of Drug Delivery Systems for Tissue Regeneration -- Nanotechnology With S-Layer Proteins -- Folding of ?-Structured Fibrous Proteins and Self-Assembling Peptides -- Application of NMR Methods to Identify Detection Reagents for Use in Development of Robust Nanosensors -- Studying 3D Subdomains of Proteins at the Nanometer Scale Using Fluorescence Spectroscopy -- Carbon Nanotubes and Nanowires for Biological Sensing -- Carbon Nanotube Systems to Communicate With Enzymes -- Molecularly Imprinted Polymers for Biomolecular Recognition -- Plasmonics-Based Nanostructures for Surface-Enhanced Raman Scattering Bioanalysis -- Bacterial Virus ?29 DNA-Packaging Motor and Its Potential Applications in Gene Therapy and Nanotechnology -- Construction of Ordered Protein Arrays -- Bioengineering and Characterization of DNA-Protein Assemblies Floating on Supported Membranes -- Nanosystems for Biosensing -- Optical Nanosensors for Detecting Proteins and Biomarkers in Individual Living Cells -- Nanoelectrodes Integrated in Atomic Force Microscopy Cantilevers for Imaging of In Situ Enzyme Activity -- Protein Amyloidose Misfolding -- Near-Field Scanning Optical Microscopy for Bioanalysis at Nanometer Resolution.
520 _aRapidly emerging at the intersection of nanotechnology, materials science, and molecular biology, the field of nanobiotechnology promises to elucidate many life processes at the molecular-level previously invisible to human inquiry, and thereby dramatically to transform diagnostics, therapy, and drug discovery in this postgenomic world. In Protein Nanotechnology: Protocols, Instrumentation, and Applications, leading experts in nanobiotechnology comprehensively review the most recent advances in instrumentation and methodology, as well as their applications in genomics and proteomics. The authors provide a wide variety of techniques and methods for dealing with protein functions and structures at the nanoscale level, including nanostructured systems, nanomaterials, carbon nanotubes and nanowires, optical nanosensors, and nanoelectrodes. Among the highlights are techniques for the in vivo tracking of biochemical processes using fluorescent molecular probes and nanosensors, and the exploration of biochemical processes and submicroscopic structures of living cells at unprecedented resolutions using near-field optics. Also discussed is the development of nanocarrier methodology for the targeted delivery of drugs whose shells are conjugated with antibodies for targeting specific antigens. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and authoritative, Protein Nanotechnology: Protocols, Instrumentation, and Applications provides investigators a wide variety of readily reproducible techniques and methods for exploring both protein functions and structures at the nanoscale level and their powerful new biological and medical applications.
700 1 _aVo-Dinh, Tuan
_eeditor literario
_0(orcid)0000-0003-3701-3326
_1https://orcid.org/0000-0003-3701-3326
_4edt
_4http://id.loc.gov/vocabulary/relators/edt
776 0 8 _iPrinted edition:
_z9781617374838
776 0 8 _iPrinted edition:
_z9781588293107
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/1592598587
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_2005
999 _c233993
_d233993