000			03957nam a22003615i 4500
001			233996
003			ES-VaUE
005			20221220020534.0
007			cr nn 008mamaa
008			170321s2017 xxu| s |||| 0|eng d
020			_a9781493968923
024	7		_a10.1007/978-1-4939-6892-3 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aTelomeres and Telomerase _bMethods and Protocols _cedited by Zhou Songyang.
250			_a3rd edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 218 páginas) _b37 ilustraciones, 18 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1587
505	0		_aIntroduction to Telomeres and Telomerase -- Analysis of Average Telomere Length in Human Telomeric Protein Knockout Cells Generated by CRISPR/Cas9 -- Telomere Length Analysis by Quantitative Fluorescent In Situ Hybridization (Q-FISH) -- Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH) -- Telomere G-Rich Overhang Length Measurement: DSN Method -- Telomere G-Overhang Length Measurement Method 2: G-Tail Telomere HPA -- Telomere Terminal G/C Strand Synthesis: Measuring Telomerase Action and C-Rich Fill-In -- Analysis of Yeast Telomerase by Primer Extension Assays -- Assessing Telomerase Activities in Mammalian Cells Using the Quantitative PCR-Based Telomeric Repeat Amplification Protocol (qTRAP) -- Telomeres and NextGen CO-FISH: Directional Genomic Hybridization (Telo-dGH™) -- Visualization of Human Telomerase Localization by Fluorescence Microscopy Techniques -- Cytogenetic Analysis of Telomere Dysfunction -- Probing the Telomere Damage Response -- Induction of Site-Specific Oxidative Damage at Telomeres by Killerred-Fused Shelretin Proteins -- Using Protein-Fragment Complementation Assays (PCA) and Peptide Arrays to Study Telomeric Protein-Protein Interactions -- In Vitro Preparation and Crystallization of Vertebrate Telomerase Subunits -- Human Telomeric G-Quadruplex Structures and G-Quadruplex-Interactive Compounds -- Analysis of Telomere-Homologous DNA with Different Conformations Using 2D Agarose Electrophoresis and In-Gel Hybridization -- Analysis of Telomere Proteins by Chromatin Immunoprecipitation (ChIP). .
520			_aThis third edition volume expands on the previous editions with new and improved assays that ask essential questions of telomere regulation in diverse model systems. The chapters in this book discuss genetic, proteomic, genomic, biochemical, and molecular approaches that provide insight into the complex protein interaction networks active in the telomere chromatin. This book also details information regarding telomere dynamics in response to stress or stimuli. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Telomeres and Telomerase: Methods and Protocols, Third Edition is a valuable and informative resource for anyone who is interested in the developing field of telomeres and telomerase.
700	1		_aSongyang, Zhou _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493968916
776	0	8	_iPrinted edition: _z9781493968930
776	0	8	_iPrinted edition: _z9781493983308
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6892-3 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c233996 _d233996