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008 100301s1993 xxu| s |||| 0|eng d
020 _a9781592595037
024 7 _a10.1385/0896032345
_2doi
040 _aES-MaUEC
_bspa
_cES-MaUEC
100 1 _aBurrell, Michael M.
_eautor
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
245 1 0 _aEnzymes of Molecular Biology
_cby Michael M. Burrell.
250 _a1st edition 1993
264 1 _aTotowa, NJ
_bHumana Press
_c1993
300 _a1 recurso en línea (X, 370 páginas)
_b
336 _atexto
_btxt
_2rdacontent
337 _aelectrónico
_bc
_2rdamedia
338 _arecurso electrónico
_bcr
_2rdacarrier
347 _aarchivo de texto
_bPDF
490 0 _aMethods in Molecular Biology
_x1940-6029
_v16
505 0 _aNucleases -- Deoxyribonuclease I (EC 3.1.21.1) and II (EC 3.1.22.1) -- DNA Polymerases (EC 2.7.7.7) -- Taq Polymerase (EC 2.7.7.7) -- Eukaryotic Nuclear RNA Polymerases (EC 2.7.7.6) -- Reverse Transcriptase (EC 2.7.7.49) -- Terminal Deoxyribonucleotidyl Transferase (EC 2.7.7.31) -- Restriction Enzymes -- DNA Methyltransferases (EC 2.1.1.72 and EC 2.1.1.73) -- DNA and RNA Ligases (EC 6.5.1.1, EC 6.5.1.2, and EC 6.5.1.3) -- The BAL 31 Nucleases (EC 3.1.11) -- Mung-Bean Nuclease 1 (EC 3.1.30.1) -- RNase A (EC 3.1.27.5) -- Pronase (EC 3.4.24.4) -- Proteolytic Enzymes for Peptide Production -- Proteinase K (EC 3.4.21.14) -- Carboxypeptidase Y (EC 3.4.16.1) -- Aminopeptidases -- Alkaline Phosphatase (EC 3.1.3.1) -- Polynucleotide Kinase (EC 2.7.1.78).
520 _aThe scientist' s understanding of the cell at the molecular level has advanced rapidly over the last twenty years. This improved understa- ing has led to the development of many new laboratory methods that increasingly allow old problems to be tackled in new ways. Thus the modern scientist cannot specialize in just one field of knowledge, but must be aware of many disciplines. To aid the process of investigation, the Methods Molecular Biology series has brought together many protocols and has highlighted the useful variations and the pitfalls of the different methods. However, protocols frequently cannot be simply taken from the shelf. Thus the starting sample for a chosen protocol may be unavailable in the correct state or form, or the products of the procedure require a different sort of processing. Therefore the scientist needs more detailed information on the nature and requirements of the enzymes being used. This information, though usually available in the literature, is often widely dispersed and frequently occurs in older volumes of journals; not everyone has comprehensive library facilities available. Also many scientists searching out such information are not trained enzymologists and may be unaware of some of the parameters that are important in a specific enzyme reaction.
776 0 8 _iPrinted edition:
_z9781489939920
776 0 8 _iPrinted edition:
_z9780896033221
776 0 8 _iPrinted edition:
_z9780896032347
856 4 0 _uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1385/0896032345
_z(usuarios Universidad Europea de Valencia)
942 _2lcc
_cLE
988 _aSpringer_Protocols_1993
999 _c234002
_d234002