000			05126nam a22003735i 4500
001			234004
003			ES-VaUE
005			20221220020535.0
007			cr nn 008mamaa
008			161202s2017 xxu| s |||| 0|eng d
020			_a9781493966738
024	7		_a10.1007/978-1-4939-6673-8 _2doi
040			_aES-MaUEC _bspa _cES-MaUEC
245	1	0	_aBacterial Pathogenesis _bMethods and Protocols _cedited by Pontus Nordenfelt, Mattias Collin.
250			_a1st edition 2017
264		1	_aNew York, NY _bSpringer International Publishing _c2017
300			_a1 recurso en línea (XI, 357 páginas) _b71 ilustraciones, 48 ilustraciones a color
336			_atexto _btxt _2rdacontent
337			_aelectrónico _bc _2rdamedia
338			_arecurso electrónico _bcr _2rdacarrier
347			_aarchivo de texto _bPDF
490	0		_aMethods in Molecular Biology _x1940-6029 _v1535
505	0		_aProtein-Based Strategies to Identify and Isolate Bacterial Virulence Factors -- Analysis of Bacterial Surface Interactions with Mass Spectrometry-Based Proteomics -- Differential Radial Capillary Action of Ligand Assay (DRaCALA) for High-Throughput Detection of Protein-Metabolite Interactions in Bacteria -- Identifying Bacterial Immune Evasion Proteins using Phage Display -- Competition for Iron between Host and Pathogen: A Structural Case Study on Helicobacter pylori -- Common Challenges in Studying the Structure and Function of Bacterial Proteins: Case Studies from Helicobacter pylori -- Development of a Single Locus Sequence Typing (SLST) Scheme for Typing Bacterial Species Directly from Complex Communities -- Reconstructing the Ancestral Relationships between Bacterial Pathogen Genomes -- Making Fluorescent Streptococci and Enterococci for Live Imaging -- Computer Vision-Based Image Analysis of Bacteria -- Assessing Vacuolar Escape of Listeria Monocytogenes -- Immobilization Techniques of Bacteria for Live Super-Resolution Imaging using Structured Illumination Microscopy -- Negative Staining and Transmission Electron Microscopy of Bacterial Surface Structures -- Detection of Intracellular Proteins by High-Resolution Immunofluorescence Microscopy in Streptococcus pyogenes -- Antibody Guided Molecular Imaging of Infective Endocarditis Infection -- The Zebrafish as a Model for Human Bacterial Infections -- Determining Platelet Activation and Aggregation in Response to Bacteria -- Killing Bacteria with Cytotoxic Effector Proteins of Human Killer Immune Cells: Granzymes, Granulysin and Perforin -- In Vitro and In Vivo Biofilm Formation by Pathogenic Streptococci -- Murine Mycobacterium marinum Infection as a Model for Tuberculosis -- Generating and Purifying Fab Fragments from Human and Mouse IgG using the Bacterial Enzymes IdeS, SpeB and Kgp -- Measuring Antibody Orientation at the Bacterial Surface -- Towards Clinical use of the IgG Specific Enzymes IdeS and EndoS against Antibody-Mediated Diseases.
520			_aThis volume discusses various methods and protocols used for the experimentation of a wide range of bacterial species, such as Streptococcus pyogenes, Staphylococcus aureus, Streptococcus pneumonia, Listeria monocytogenes, and Mycobacterium marinum. Bacterial Pathogens: Methods and Protocols is divided into 6 parts: Part 1 describes different approaches to identifying and characterizing bacterial effector molecules; Part 2 deals with structural biology of bacterial pathogenesis and how to overcome folding and stability problems with recombinantly expressed proteins; Part 3 details methodology that identifies bacteria in complex communities and how genomes of bacterial pathogens have evolved; Part 4 reflects on the rapid development of advanced imaging techniques that address questions about molecular properties of individual live bacteria, ultrastructure of surfaces, and subcellular localization of bacterial proteins; Part 5 describes methods from in vitro and in vivo modeling of bacterial infections; and Part 6 explores how bacterial pathogens are the true experts of the immune system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Bacterial Pathogens: Methods and Protocols is a valuable resource for anyone who is interested in this fascinating and evolving field.
700	1		_aNordenfelt, Pontus _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
700	1		_aCollin, Mattias _eeditor literario _4edt _4http://id.loc.gov/vocabulary/relators/edt
776	0	8	_iPrinted edition: _z9781493966714
776	0	8	_iPrinted edition: _z9781493966721
776	0	8	_iPrinted edition: _z9781493982684
856	4	0	_uhttps://go.openathens.net/redirector/universidadeuropea.es?url=https://doi.org/10.1007/978-1-4939-6673-8 _z(usuarios Universidad Europea de Valencia)
942			_2lcc _cLE
988			_aSpringer_Protocols_2017
999			_c234004 _d234004